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Electrophoresis

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For Tissue Dissociation
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Life Sciences

From Cells to Molecules

Enzymology

Enzymes, Substrates, Inhibitors, Detection

Microscopy

Fixation, Embedding, Staining

Ion Exchange Media

For Bioseparation Product Center

 

Partner Catalogs
Hoefer Inc., USA

Equipment for Electrophoresis

Elchrom Scientific, CH

Equipment & Gels for DNA Electrophoresis

BioCision, USA

Standardizing Samples

Delphi™ Genetics SA

Cloning & Expression
in Bacteria

IMPLEN GmbH, D

NanoPhotometer
for Small Volumes

Isogen Life Science B.V.

Proxima Imaging Platform

Generon Ltd., UK

GST-tagged Protein Purification

Pro-Chem Inc., USA

Antibody Purification

 

HomeNew Products Electrophoresis

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SERVA FastLoad DNA Ladders

FastLoad.jpg

The DNA ladders are ready-to-use in loading buffer and can be stored for 6 months at 25 °C (except cat. no. 39215) or for 12 months at 4 °C — no thawing, further dilution or adding of buffer components is necessary before use. At -20 °C they are stable for at least two years.
For estimation of DNA mass of bands of similar size with comparable intensity, the approximate mass of each marker band is indicated.

SERVA FastLoad 50 bp DNA ladder
17 fragments from 50 – 1500 base pairs. The bands at 200 bp and 500 bp have increased intensity and serve as reference points.

SERVA FastLoad 100 bp DNA ladder
12 fragments from 100 – 3000 base pairs. The bands at 500 bp and 1500 bp have increased intensity and serve as reference points.

SERVA FastLoad 1 kb DNA ladder
14 fragments from 250 – 25000 base pairs. The bands at 1000 bp and 3000 bp have increased intensity and serve as reference points.

39315 SERVA FastLoad 50 bp DNA ladder
39316 SERVA FastLoad 100 bp DNA ladder
39317 SERVA FastLoad 1 kb DNA ladder

SERVA Triple and Pink Color Protein Standards

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The protein standards are ready-to-use — no heating, further dilution or adding of reducing reagents is necessary before use. The markers are stable for up to two weeks at 25 °C , for up to 3 months at 4 °C and up to two years at -20 °C . They are designed for monitoring protein separation during SDS PAGE, verification of Western Blotting transfer efficiency and for approximate sizing of proteins.

SERVA Triple Color Protein Standard II
Mixture of 10 pre-stained proteins of a molecular weight range from 11 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for one green band at 25 kDa and one red band at 75 kDa.

SERVA Triple Color Protein Standard III
Mixture of 13 pre-stained proteins of a molecular weight range from 5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for one green band at 25 kDa and one red band at 75 kDa.

SERVA Pink Color Protein Standard II
Mixture of 11 pre-stained proteins of a molecular weight range from 10.5 to 175 kDa. Proteins are covalently coupled with a pink chromophore and contains for easy identification three blue bands at approximate 10 kDa, 40 kDa and 90 kDa.

39257 SERVA Triple Color Standard II
39258 SERVA triple Color Protein Standard III
39259 SERVA Pink Color Protein Standard II

SERVA HPE™ Lightning Red

Fluorescent Pre-labeling of Proteins in 2D PAGE

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Fluorescent dye for rapid labelling of proteins prior to 1D SDS or 2D PAGE, making any staining and washing steps after electrophoresis unnecessary. Moreover, although sensitivity is at least as high as silver staining or other fluorescence dyes, costs are significantly lower.
The labelling procedure is simple and quick and does not require any additional measures like clean-up or centrifugation steps.
In contrast to other fluorescent dyes used for labelling lysine residues of proteins, SERVA HPE™ Lightning Red is compatible with all additives typically used for sample solubilisation and protein extraction, including carrier ampholytes and reductants. Furthermore, no pH adjustment is necessary, alkalescent conditions are sufficient

SERVA HPE™ Lightning Red has a number of advantages:

  • Direct detection
  • No staining and washing steps after the run
  • Very high sensitivity, < 100 pg protein / spot
  • Wide dynamic and linear range of > 104
  • No over-staining effects
  • Fully MS compatible
  • After imaging gel can be further processed by Western blotting
43400 SERVA HPE™ Lightning Red

SERVA Lightning Red for 1D SDS PAGE

Fluorescent Pre-labelling of Proteins in 1D SDS PAGE

Lightning-Red.jpg

Fluorescent dye for rapid labelling of proteins prior to 1D SDS PAGE, making any staining and washing steps after electrophoresis unnecessary. Kits are sufficient for
500 lanes or 1250 lanes – no matter what size of gel (mini, wide or large format) you use. Increase detection sensitivity to the level of silver staining with costs comparable to Coomassie® staining.
The labelling procedure is simple and quick. Samples of protein concentrations between 1 and 10 mg/ml can be used and no purification or concentration steps after labelling are necessary.

SERVA Lightning Red has a number of advantages:

  • Direct detection at low costs
  • No staining and washing steps after the run
  • Very high sensitivity
  • Wide dynamic and linear range
  • No over-staining effects
  • Fully MS compatible
  • After imaging gel can be further processed by Western blotting
43401 SERVA Lightning Red

SERVA Purple

A well-established fluorescent dye for 1D and 2D PAGE
with improved performance

SERVA-Purple.jpg

The fluorescent dye Deep Purple (marketed by GE) and LavaPurple (marketed by Gelcompany and SERVA) has been used by many laboratories for staining proteins in gels and on blotting membranes.
The dye is based on a small naturally occurring fluorescent compound “Epicocconone” that reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product. SERVA Purple is a further development of this compound “Epicocconone”. It has improved properties due to a novel production and purification technology by unchanged cost-effectiveness compared to other fluorescence dyes or even silver staining.
The staining protocols used for Deep Purple and LavaPurple, can be applied without any alteration. Comparative staining tests within SERVA and in some customer laboratories have produced excellent results, absolutely comparable to results achieved with the predecessor Epicocconone dyes.

43386 SERVA Purple

SERVA ProteinStain Fluo-Y

Fast and sensitive fluorescent staining of 1 D and 2D gels
directly detectable by UV-imaging systems

After fixation gels are directly stained with SERVA ProteinStain Fluo-Y. The whole staining procedure is completed in as little as 30 min. A destain step is usually not necessary. The bound dye emits a strong fluorescence of bright golden colour with a maximum emission wave length near 570 nm. Stained gels may be therefore directly visualized with a variety of different UV-based fluorescence imaging systems.
Increase your detection sensitivity to the level of fluorescence staining without the need of investment into expensive equipment.

  • Highly sensitive fluorescent protein stain over a wide dynamic range
  • Two-step protocol, no destain
  • Directly detected by UV-imaging systems
  • MS compatible - dye is removable from gel by washing with water
35092 SERVA ProteinStain Fluo-Y

SERVA BlueShake 3D

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Due to the large incubation platform of 350 x 350 mm and the smooth 3D motion (including start and stop) even large sized gels can be stained/destained without breaking the gel.
The 3D Shaker is equipped with a digital LED display which shows rotational speed and timer function. Via the user-friendly control panel the rotational speed and time can be manually set. Due to its very low noise level the device is suited for continuous operation.

  • Highest flexibility: large platform for individual customer needs
  • Quiet operation, suitable for long term work
  • Excellent price/performance ratio
  • Via touch panel programmable interface
  • Rock solid metal housing
  • Smart design - made in Germany
SERVA BlueShake 3D

BlueVertical™ PRiME™ - the new mini slab gel system from SERVA

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BlueVertical™ PRiME™ has been especially designed to run SERVAGel™ precast gels and other precast gels with an outer cassette dimension of 10 x 10 x 0.7 cm.
The fixture of the inner core unit has been completely re-engineered  to ensure the precise fit of the gel cassette(s). The BlueVertical™ is the perfect electrophoresis mini tank system to run precast gels in SDS PAGE, native PAGE, IEF or nucleic acid PAGE applications. A unique innovative clamp system keeps the gel cassettes in their correct place at the inner core running module, leak-free and ready to start within seconds. Built-in convenient operation features result in improved daily work.
Developed, produced and assembled in Germany – another top quality product from SERVA.

  • Superb results
  • Top resolution
  • Easy and safe to handle
  • Unique, leak-free clamp system
  • Ergonomic, compact design
  • For SERVAGel™ precast gels
BlueVertical™ PRiME™ (BV 104)

SERVAGel™ TG PRiME™

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New Vertical Precast Tris-Glycine Gels for Standard SDS PAGE according to Laemmli:

  • Long shelf life (12 months from production)
  • High resolution with excellent band sharpness in only approx. 35 min
  • Use your standard Tris/Glycine buffer system for electrophoresis and blotting
  • Available as homogeneous as well as gradient gel with 10 or 12 sample wells, as 2D gel (12 %, 14 %, gradient 8-16 %)
  • Ready-to-use precast gels in unbreakable plastic cassette (10 x 10 cm), compatible with most commercially available slab gel tanks
SERVAGel™ Precast gels - Overview

SERVALight HRP Chemiluminescence Kits -
A New Family of Substrates

Four different types of highly sensitive ready-to-use kits for chemiluminescence detection of membrane bound antigens or nucleic acid sequences, labeled directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies/streptavidin.

SERVALight Polaris CL HRP WB Substrate Kit

  • High sensitivity, low pictogram limit of detection
  • Long light emission for 6 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:1000 -1:5000
    Secondary antibody dilution 1:20.000 – 1:100.000

 SERVALight Eos CL HRP WB Substrate Kit

  • Very high sensitivity, high femtogram limit of detection
  • Very long light emission for 12 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:1000 -1:15000
    Secondary antibody dilution 1:25.000 – 1:150.000

 SERVALight EosUltra CL HRP WB Substrate Kit

  • Ultrahigh sensitivity, low femtogram limit of detection
  • extremely long light emission at a very high signal level for 18 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:5000 -1:50000
    Secondary antibody dilution 1:50.000 – 1:250.000

SERVALight Helios CL HRP WB Substrate Kit

  • Utmost sensitivity, low femtogram limit of detection
  • Long light emission for 8 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:5000 -1:100000
    Secondary antibody dilution 1:100.000 – 1:500.000
42584 SERVALight Polaris
42585 SERVALight Eos
42586 SERVALight EOSUltra
42587 SERVALight Helios

Bio-5000 VIS Gel Scanner

Bio-5000 Professional VIS Gel Scanner for SDS PAGE gels,
Western Blots etc.

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Microtek‘s Bio-5000, performing advanced application in biological laboratory, is perfectly designed for image capture of dry or wet samples of gel electrophoresis, SDS-PAGE, IEF gels, western blotting films, documents and so on. The Bio-5000, presenting greater image quality, has great features including 4800 optical resolution, 48-bit color scanning mode and linearity over 4.0 O.D.

  • Designed removable waterproof glass holder
  • Deliver the accurate images no matter the scanned target
    is dry or wet gel samples
  • Make batch scanning easily
  • Mechanism designed of Microtek patented E.D.I.T. technology

 Specification:

 Scanning
 Modes
 Color and grayscale, single scanning pass
 True 48-bit-color
 16-bit grayscale (65,536 shades of gray)
 Scanning
 Area
 Reflective: max. 216 mm x 356 mm
 Transparency: max. 216 mm x 260 mm
 Linearity  4.0 O.D.
 Resolution  4800 dpi x 9600 dpi
 Interface  Hi-Speed USB (USB 2.0)
 Dimension  567 mm x 385 mm x 158 mm (L x W x H)
 Weight  12 kg
 Voltage  AC 100 V to 240 V; 50 - 60 Hz
 Power  30 W
Bio-5000 VIS Gel Scanner

HPE™ - High Performance Electrophoresis

The new multi-level electrophoresis system for up to four high resolution
1D and 2D separations in flatdbed gels!

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The HPE™ FlatTop Tower and the HPE™ gels have been developed together as a system, in order to achieve better results than can be obtained with conventional SDS polyacrylamide gel electrophoresis (PAGE) technology.

The Equipment: HPE™ Tower System

The Flatbed Gels: 2D SDS PAGE Gel Kits

The Flatbed Gels: 1D SDS PAGE Gel Kits

The Flatbed Gels: FocusGels for Isoelectric Focusing (IEF)

The Vertical Gels: Precast 2D Vertical Gel Kits

 

The HPE™ Tower System
The SERVA HPE™ tower consists of four horizontal electrophoresis chambers, which are built as movable drawers into a metal housing. The flatbed gels are placed on aluminium oxide ceramics cooling plates, which ensures very efficient heat dissipation and therefore straight electrophoresis migration in each gel. The HPE™ tower also has a built-in pump for optimum cooling water flow through the plates. When needed, electrophoresis buffer is supplied by fibre wicks, soaked with concentrated buffer. The electrode positions are adjustable to different gel sizes. The HPE™ tower runs with an external power supply (HPE-PSP) and a thermostatic circulator (cat. No. HPE-CU1), both included with the HPE™ tower system (HPE-TS1).
BlueLine Tower System - Products

The Flatbed Gels
To complete the HPE technology flatbed gels for different applications are available:

2D SDS PAGE Gel Kits
2D SDS Page gel kits are available in three different formats:

• HPE Large Format Gels: suitable for running 1 x 24 cm IPG Strip + marker lane
• HPE Double Gel Kits: suitable for running 2 x 11 cm IPG Strip + marker lane
• HPE Triple Gel Kits: suitable for running 3 x 7 cm IPG Strip + marker lane

All gels can be run by horizontal electrophoresis on HPE™ Tower and BH-2C. Gels are on standard backing for Silver or Coomassie staining or on non-fluorescence (NF) backing for best results with fluorescent staining and labeling. Each kit contains 4 gels + buffer kit (including running and equilibration buffer, buffer wicks and cooling contact fluid).
HPE Precast 2D Flatbed  Gels - Products

1D SDS PAGE Gel Kits
There are different gel types and application optimized buffer kits available:

1D SDS PAGE gel kits are ready to use kits for SDS PAGE. The gel concentration of the gels are 10, 12.5 or 15 % (T) acrylamide. They are available with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. Each kit contains 4 gels + buffer kit. All gels can be run by horizontal electrophoresis on HPE™ Tower and BH-2C. 

CleanGels are rehydratable gels are available with acrylamide content of 10 or
15 % (T) and with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. A special formulated ampholyte mixture for CSF analysis on CleanGels is available.
•   Sortex Kit for Cereal-cultivar-differentiation-electrophoresis. Contains 4 gels on standard backing and special buffer kit for flatbed electrophoresis on HPE™ tower and BH-2C.
Native Buffer Kit contains buffer system for native cathodal flatbed electrophoresis of proteins on HPE tower and BH-2C.
SDS Neutral Buffer Kit contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks for flatbed electrophoresis on HPE tower and BH-2C.
SDS Borate Buffer Kit for flatbed SDS PAGE of high molecular weight proteins on CleanGels. Contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks.

CleanGels  Products

The Precast 2D Vertical Gel Kits
The 2D DALT gel kits contain 6 or 12 gels for ETTAN DALTsix and ETTAN DALTtwelve, respectively. They contain 6 or 12 gels + buffer kits containing anodal and cathodal buffers as well as IPG equilibration buffer and agarose for strip fixation.
Precast 2D Vertical Gel Kits - Products

Flatbed Gels for Isoelectric Focusing (IEF)
The FocusGels are ready-tp-use gels for flatbed isoelectric focusing on HPE™ Tower and BH-2C. They are available in 9 different pH ranges. The gels have none, 24 (type 24S with 25 µl/slot) or 40 (type 40S with 12 µl/slot) slots.
FocusGels - Products

Thursday, August 28, 2014

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SERVA News


Markthalle:
Juli - August 2014


Hier geht es zur Aktion

New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)


 more

The SERVA Catalog 2014
is online!


Hier geht es zur Aktion

 more

SERVA Promotions VIII/2014



Markthalle Juli - August 

Produkt des Monats August
(D + A)

Verleihung des Transferpreises 2013/2014
der WWU Münster


an Prof. Dr. Simone König und SERVA Electrophoresis GmbH

 more

New Brochure:
Instruments for Life Sciences Research!


 more

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Markthalle:
Juli - August 2014

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Hier geht es zur Aktion

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New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

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ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.


SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP

The SERVA Catalog 2014
is online!

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Hier geht es zur Aktion

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• Biochemicals A-Z

• Sample Preparation

• Electrophoresis

• Collagenase & Neutral Protease

• Life Sciences

• Enzymology

• Microscopy

• Ion Exchange Media

 


Find the SERVA Online Catalog 2014 here!

SERVA Promotions VIII/2014

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Markthalle Juli - August 

Produkt des Monats August
(D + A)

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Verleihung des Transferpreises 2013/2014
der WWU Münster

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an Prof. Dr. Simone König und SERVA Electrophoresis GmbH

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Die Chemikerin Prof. Dr. Simone König erhielt am 3. Juni 2014 den
mit 20.000 € dotierten Transferpreis der Westfälischen Wilhelms-Univer-
sität (WWU) für die Verbesserung einer in der Labortechnik sehr häufig eingesetzten Methode der
Gel-Elektrophorese.

Geehrt wurde außerdem Günter Theßeling von der Firma SERVA Electrophoresis GmbH in Heidelberg, die die Entwicklung jetzt auf den Markt bringt.

Film: Von der Idee zum Mercator-Gel


Verleihung des WWU-Transferpreises 2013/2014.pdf

New Brochure:
Instruments for Life Sciences Research!

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Electrophoresis Systems &
Laboratory Equipment:


SERVA Brochure: Instruments for Life Sciences Research (pdf)

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