Electrophoresis

SERVA BlueBlot Semi-Dry Blotter

The BlueBlot semi-dry blotter forms a homogeneous electrical field that guarantees fast and efficient transfer of proteins from gel to membrane.

It is available with blotting areas of 11 cm x 11 cm, 17 cm x 17 cm and 24 cm x 26 cm.  For the two smaller units, both electrode pairs (11 cm and 17 cm) will fit into the same base housing, what makes the blotter very flexible for small and larger gels.

With the 24 cm x 26 cm unit large gels like the horizontal HPE™ 2D gels can be blotted.

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The key features:

  • Platinum-covered steel net as anode
  • Stainles steel plate as cathode
  • Deployable for thicker gels and blotting stacks
  • Blotting area from 11 cm x 11 cm up to 24 cm x 26 cm

SERVA Unstained Protein Standard II

  • Mixture of 9 recombinant proteins (12 kDa to 120 kDa)
  • Ready-to-use, no reconstitution,
    further dilution or heating required
  • 50 kDa band with double intensity as landmark
    for easy allocation of molecular weights
  • 12 kDa prestained band for monitoring electrophoresis
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SERVA VisiBlot Standard I

  • Mixture of 10 recombinant proteins (25 kDa to 150 kDa)
  • Prestained bands (25 kDa, 45 kDa, 85 kDa) for monitoring electrophoresis and membrane transfer
  • Accurate molecular weight determination of proteins detected on transfer membrane
  • Ready-to-use, no reconstitution, further dilution or heating required
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  • Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods

SERVAGel™ TG PRiME™

  • Precast Tris-Glycine mini vertical gels with long shelf life (12 months from production)
  • Now available with 15 wells (8 %, 10 %, 12 %, 14 %, Gradient 4-12 %, 4-20 % und 8 - 16 %)
  • Sample volume: 20 µl (50 µl with 10 well - and 35 µl with 12 well comb)
  • High resolution with excellent band sharpness in only approx. 35 min
SERVAGel TGLS

Xpress Blotting Kit

  • Fast and efficient transfer of high and low molecular weight proteins in only 15 min
  • Blotting Fleece instead of several layers of blotting paper for undisturbed transfer
  • Ready-to-use – no preparing of buffer or cutting
    of blotting papers or membranes
  • Compatible with nitrocellulose and PVDF membranes
Xpress-blotting-kit.jpg

GeneGnome XRQ

The GeneGnome XRQ is dedicated to chemiluminescence imaging. The system is built for high performance and automation and features a next generation high quantum efficiency CCD camera for even greater sensitivity. A very simple set-up process means a single click will automatically capture a quality image of any Western blot.

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  •  Optimized for imaging chemiluminesence Western blots
  •  Sensitive, high quantum efficiency cooled CCD camera (73 % @ 425 nm)
  •  Blot size 11 cm x 8 cm
  •  Small footprint (w 38 cm, d 39 cm, h 46 cm, 11.15 kg)
  •  Available in three variants:
    o GeneGnome XRQ complete with monitor and built-in processor
    o GeneGnome XRQ-NM complete with built-in processor but no monitor
    o GeneGnome XRQ-NPC with no monitor or built-in processor

SERVA BlueLight Table

The SERVA BlueLight Table consists of a blue light LED light source (470 nm, good for 30,000 h) and an amber filter that serves as lid. The viewing area is 20 x 12 cm.
DNA Gels (agarose, acrylamide) stained with DNA Stain Clear G or similar could be detected as well as protein bands in gels stained with fluorescence stains like SERVA Lightning Red.

BLueLight Table.jpg

A cardboard hood to snapshot your gel with a smartphone is delivered with the table as well as a gel cutting knife to cut out target from the gel for further experiments.

  • For DNA and protein band detection in gels
  • Blue light LED transiluminator at 470 nm
  • 4° ergonomic viewing angle
  • Gel size up to 20 x 12 cm
  • Mini cardboard darkroom for a Smartphone snapshot of your gel
  • Weight < 1.3 kg

SERVA BlueImager

The SERVA BlueImager is equipped with a CCD camera, a five-fold filter changer and an RGB table with a gel area of 25.5 x 19.5 cm for documentation of even very large protein gels (1D/2D).
After pre-labelling your protein samples immediate documentation of the gel is done without staining/washing steps. The broad fluorescence detection includes SERVA Purple stained gels as well as multiplexed gels (e.g. DIGE).

BlueImager.jpg

The white light table allows the imaging of Coomassie or silver stained gels.
The system is easily operated via a built-in touch screen or via WiFi by your smartphone or tablet.

  • Versatile applications in fluorescence detection
  • For large format gels
  • Time-saving in combination with SERVA Lightning Red
  • Highly flexible due to 5-fold filter changer and RGB LEDs
  • High sensitivity (1 ng)

SERVA BlueCube 300

The SERVA BlueCube 300 is equipped with a CMOS camera, a built-in UV transilluminator, a filter changer including an UV filter, as well as a gel capturing and analysis software (GelView 1D).
It is suitable for documentation of DNA gels stained with ethidium bromide, but also for gels stained with DNA Stain Clear G or similar.
A magnetic shield protects the user when cutting out DNA bands from the gel. With dimensions of 30 x 26 x 24 cm and a weight of 10.4 kg only, it is a very compact and bench place saving system.

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  • Integrated UV transilluminator
  • Gel area is 18 x 14 cm
  • Automatic UV light shut-off
  • High performance and easy handling system at a low price
  • With the SERVA BlueCube 300L version, an external computer is included

SERVA Lightning SciDyes

  • Designed for minimal labelling prior to protein detection in DIGE
  • Size and charge matched fluorescence dyes
  • Compatible with all imagers suitable for detection of Cy2®, Cy3® and Cy5®.
  • Gels labelled with SERVA Lightning SciDyes are ready for subsequent mass spectrometry analysis
SERVALightning.jpg

Cy2, Cy3 and Cy5 = trademarks of GE Healthcare Company


Agarose SERVA 3 : 1

  • Alternative for NuSieve® agarose for high resolution of small
    (10 bp - 1000 bp) DNA, RNA and PCR fragments
  • High purity for low background even at high gel concentrations (up to 6 % in 1x TAE buffer)
  • Optimized gel strength for easy-to-handle gels
  • Tested for applications in molecular biology
Agarose_SERVA.jpg

NuSieve® = trademark of the company Lonza


Agarose SERVA FastSolve Tablets

  • Fast dissolving agarose tablets delivered in a convenient blister pack
  • Made of standard melting point agarose for high resolution of DNA and RNA (100 bp – 60 kb)
  • Gels of high clarity and low background
  • Hands-on-time to
  • Optimized gel strength for easy-to-handle gels
  • Tested for applications in molecular biology
Agarose_SERVA.jpg

SERVALight Vega CL HRP WB Substrate Kit

Directly compatible with protocols of standard ECL western blot substrates of other vendors – no change of protocol but superior performance and less costs  

 

SERVA Light Vega Competitor.jpg
  • High signal intensity, low pictogram limit of detection
  • Low background even after long exposures
  • High signal/noise ratio
  • Working solution is stable for minimum 5 days – reproducible results, less waste
  • Primary antibody dilution 1:100 -1:5000
  • Secondary antibody dilution 1:1000 – 1:15.000

SERVA FastLoad DNA Ladders

The DNA ladders are ready-to-use in loading buffer and can be stored for 6 months at 25 °C (except cat. no. 39215) or for 12 months at 4 °C — no thawing, further dilution or adding of buffer components is necessary before use. At -20 °C they are stable for at least two years.
For estimation of DNA mass of bands of similar size with comparable intensity, the approximate mass of each marker band is indicated.

SERVA FastLoad 50 bp DNA ladder
17 fragments from 50 – 1500 base pairs. The bands at 200 bp and 500 bp have increased intensity and serve as reference points.

SERVA FastLoad 100 bp DNA ladder
12 fragments from 100 – 3000 base pairs. The bands at 500 bp and 1500 bp have increased intensity and serve as reference points.

FastLoad.jpg

SERVA FastLoad 1 kb DNA ladder
14 fragments from 250 – 25000 base pairs. The bands at 1000 bp and 3000 bp have increased intensity and serve as reference points.


SERVA Triple and Pink Color Protein Standards

The protein standards are ready-to-use — no heating, further dilution or adding of reducing reagents is necessary before use. The markers are stable for up to two weeks at 25 °C , for up to 3 months at 4 °C and up to two years at -20 °C . They are designed for monitoring protein separation during SDS PAGE, verification of Western Blotting transfer efficiency and for approximate sizing of proteins.

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SERVA Triple Color Protein Standard II
Mixture of 10 pre-stained proteins of a molecular weight range from 11 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for one green band at 25 kDa and one red band at 75 kDa.

SERVA Triple Color Protein Standard III
Mixture of 13 pre-stained proteins of a molecular weight range from 5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for one green band at 25 kDa and one red band at 75 kDa.

SERVA Pink Color Protein Standard II
Mixture of 11 pre-stained proteins of a molecular weight range from 10.5 to 175 kDa. Proteins are covalently coupled with a pink chromophore and contains for easy identification three blue bands at approximate 10 kDa, 40 kDa and 90 kDa.


SERVA HPE™ Lightning Red

Fluorescent Pre-labeling of Proteins in 2D PAGE

Fluorescent dye for rapid labelling of proteins prior to 1D SDS or 2D PAGE, making any staining and washing steps after electrophoresis unnecessary. Moreover, although sensitivity is at least as high as silver staining or other fluorescence dyes, costs are significantly lower.
The labelling procedure is simple and quick and does not require any additional measures like clean-up or centrifugation steps.

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In contrast to other fluorescent dyes used for labelling lysine residues of proteins, SERVA HPE™ Lightning Red is compatible with all additives typically used for sample solubilisation and protein extraction, including carrier ampholytes and reductants. Furthermore, no pH adjustment is necessary, alkalescent conditions are sufficient.

SERVA HPE™ Lightning Red has a number of advantages:

  • Direct detection
  • No staining and washing steps after the run
  • Very high sensitivity, < 100 pg protein / spot
  • Wide dynamic and linear range of > 104
  • No over-staining effects
  • Fully MS compatible
  • After imaging gel can be further processed by Western blotting

SERVA Lightning Red for 1D SDS PAGE

Fluorescent Pre-labelling of Proteins in 1D SDS PAGE

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Fluorescent dye for rapid labelling of proteins prior to 1D SDS PAGE, making any staining and washing steps after electrophoresis unnecessary. Kits are sufficient for
500 lanes or 1250 lanes – no matter what size of gel (mini, wide or large format) you use. Increase detection sensitivity to the level of silver staining with costs comparable to Coomassie® staining.
The labelling procedure is simple and quick. Samples of protein concentrations between 1 and 10 mg/ml can be used and no purification or concentration steps after labelling are necessary.

SERVA Lightning Red has a number of advantages:

  • Direct detection at low costs
  • No staining and washing steps after the run
  • Very high sensitivity
  • Wide dynamic and linear range
  • No over-staining effects
  • Fully MS compatible
  • After imaging gel can be further processed by Western blotting

SERVA Purple

A well-established fluorescent dye for 1D and 2D PAGE with improved performance

The fluorescent dye Deep Purple (marketed by GE) and LavaPurple (marketed by Gelcompany and SERVA) has been used by many laboratories for staining proteins in gels and on blotting membranes.

SERVA-Purple.jpg

The dye is based on a small naturally occurring fluorescent compound “Epicocconone” that reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product. SERVA Purple is a further development of this compound “Epicocconone”. It has improved properties due to a novel production and purification technology by unchanged cost-effectiveness compared to other fluorescence dyes or even silver staining.
The staining protocols used for Deep Purple and LavaPurple, can be applied without any alteration. Comparative staining tests within SERVA and in some customer laboratories have produced excellent results, absolutely comparable to results achieved with the predecessor Epicocconone dyes.


SERVA ProteinStain Fluo-Y

Fast and sensitive fluorescent staining of 1 D and 2D gels
directly detectable by UV-imaging systems

After fixation gels are directly stained with SERVA ProteinStain Fluo-Y. The whole staining procedure is completed in as little as 30 min. A destain step is usually not necessary. The bound dye emits a strong fluorescence of bright golden colour with a maximum emission wave length near 570 nm. Stained gels may be therefore directly visualized with a variety of different UV-based fluorescence imaging systems.
Increase your detection sensitivity to the level of fluorescence staining without the need of investment into expensive equipment.

  • Highly sensitive fluorescent protein stain over a wide dynamic range
  • Two-step protocol, no destain
  • Directly detected by UV-imaging systems
  • MS compatible - dye is removable from gel by washing with water

SERVA BlueShake 3D

Due to the large incubation platform of 350 x
350 mm and the smooth 3D motion (including start and stop) even large sized gels can be stained/destained without breaking the gel.
The 3D Shaker is equipped with a digital LED display which shows rotational speed and timer function. Via the user-friendly control panel the rotational speed and time can be manually set. Due to its very low noise level the device is suited for continuous operation.

SERVA BlueShake 3D.jpg
  • Highest flexibility: large platform for individual customer needs
  • Quiet operation, suitable for long term work
  • Excellent price/performance ratio
  • Via touch panel programmable interface
  • Rock solid metal housing
  • Smart design - made in Germany

BlueVertical™ PRiME™ - the new mini slab gel system from SERVA

BlueVertical™ PRiME™ has been especially designed to run SERVAGel™ precast gels and other precast gels with an outer cassette dimension of 10 x 10 x 0.7 cm.
The fixture of the inner core unit has been completely re-engineered  to ensure the precise fit of the gel cassette(s). The BlueVertical™ is the perfect electrophoresis mini tank system to run precast gels in SDS PAGE, native PAGE, IEF or nucleic acid PAGE applications.

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A unique innovative clamp system keeps the gel cassettes in their correct place at the inner core running module, leak-free and ready to start within seconds. Built-in convenient operation features result in improved daily work.
Developed, produced and assembled in Germany – another top quality product from SERVA.

  • Superb results
  • Top resolution
  • Easy and safe to handle
  • Unique, leak-free clamp system
  • Ergonomic, compact design
  • For SERVAGel™ precast gels

SERVALight HRP Chemiluminescence Kits - A New Family of Substrates

Four different types of highly sensitive ready-to-use kits for chemiluminescence detection of membrane bound antigens or nucleic acid sequences, labeled directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies/streptavidin.

SERVALight Polaris CL HRP WB Substrate Kit

  • High sensitivity, low pictogram limit of detection
  • Long light emission for 6 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:1000 -1:5000
    Secondary antibody dilution 1:20.000 – 1:100.000

 SERVALight Eos CL HRP WB Substrate Kit

  • Very high sensitivity, high femtogram limit of detection
  • Very long light emission for 12 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:1000 -1:15000
    Secondary antibody dilution 1:25.000 – 1:150.000

 SERVALight EosUltra CL HRP WB Substrate Kit

  • Ultrahigh sensitivity, low femtogram limit of detection
  • extremely long light emission at a very high signal level for 18 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:5000 -1:50000
    Secondary antibody dilution 1:50.000 – 1:250.000

SERVALight Helios CL HRP WB Substrate Kit

  • Utmost sensitivity, low femtogram limit of detection
  • Long light emission for 8 hours
  • Saves money and precious antibodies due to high dilution of antibodies:
    Primary antibody dilution 1:5000 -1:100000
    Secondary antibody dilution 1:100.000 – 1:500.000

HPE™ - High Performance Electrophoresis

The new multi-level electrophoresis system for up to four high resolution
1D and 2D separations in flatdbed gels!
The HPE™ FlatTop Tower and the HPE™ gels have been developed together as a system, in order to achieve better results than can be obtained with conventional SDS polyacrylamide gel electrophoresis (PAGE) technology.

HPE-Tower.jpg

The Equipment: HPE™ Tower System

The Flatbed Gels: 2D SDS PAGE Gel Kits

The Flatbed Gels: 1D SDS PAGE Gel Kits

The Flatbed Gels: FocusGels for Isoelectric Focusing (IEF)

The Vertical Gels: Precast 2D Vertical Gel Kits

 

The HPE™ Tower System
The SERVA HPE™ tower consists of four horizontal electrophoresis chambers, which are built as movable drawers into a metal housing. The flatbed gels are placed on aluminium oxide ceramics cooling plates, which ensures very efficient heat dissipation and therefore straight electrophoresis migration in each gel. The HPE™ tower also has a built-in pump for optimum cooling water flow through the plates. When needed, electrophoresis buffer is supplied by fibre wicks, soaked with concentrated buffer. The electrode positions are adjustable to different gel sizes. The HPE™ tower runs with an external power supply (HPE-PSP) and a thermostatic circulator (cat. No. HPE-CU1), both included with the HPE™ tower system (HPE-TS1).
BlueLine Tower System - Products

The Flatbed Gels
To complete the HPE technology flatbed gels for different applications are available:

2D SDS PAGE Gel Kits
2D SDS Page gel kits are available in three different formats:

• HPE Large Format Gels: suitable for running 1 x 24 cm IPG Strip + marker lane
• HPE Double Gel Kits: suitable for running 2 x 11 cm IPG Strip + marker lane
• HPE Triple Gel Kits: suitable for running 3 x 7 cm IPG Strip + marker lane

All gels can be run by horizontal electrophoresis on HPE™ Tower and BH-2C. Gels are on standard backing for Silver or Coomassie staining or on non-fluorescence (NF) backing for best results with fluorescent staining and labeling. Each kit contains 4 gels + buffer kit (including running and equilibration buffer, buffer wicks and cooling contact fluid).
HPE Precast 2D Flatbed  Gels - Products

1D SDS PAGE Gel Kits
There are different gel types and application optimized buffer kits available:

1D SDS PAGE gel kits are ready to use kits for SDS PAGE. The gel concentration of the gels are 10, 12.5 or 15 % (T) acrylamide. They are available with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. Each kit contains 4 gels + buffer kit. All gels can be run by horizontal electrophoresis on HPE™ Tower and BH-2C. 

CleanGels are rehydratable gels are available with acrylamide content of 10 or
15 % (T) and with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. A special formulated ampholyte mixture for CSF analysis on CleanGels is available.
•   Sortex Kit for Cereal-cultivar-differentiation-electrophoresis. Contains 4 gels on standard backing and special buffer kit for flatbed electrophoresis on HPE™ tower and BH-2C.
Native Buffer Kit contains buffer system for native cathodal flatbed electrophoresis of proteins on HPE tower and BH-2C.
SDS Neutral Buffer Kit contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks for flatbed electrophoresis on HPE tower and BH-2C.
SDS Borate Buffer Kit for flatbed SDS PAGE of high molecular weight proteins on CleanGels. Contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks.

CleanGels  Products

The Precast 2D Vertical Gel Kits
The 2D DALT gel kits contain 6 or 12 gels for ETTAN DALTsix and ETTAN DALTtwelve, respectively. They contain 6 or 12 gels + buffer kits containing anodal and cathodal buffers as well as IPG equilibration buffer and agarose for strip fixation.
Precast 2D Vertical Gel Kits - Products

Flatbed Gels for Isoelectric Focusing (IEF)
The FocusGels are ready-tp-use gels for flatbed isoelectric focusing on HPE™ Tower and BH-2C. They are available in 9 different pH ranges. The gels have none, 24 (type 24S with 25 µl/slot) or 40 (type 40S with 12 µl/slot) slots.
FocusGels - Products

Aktionen

Product of the Month September:

Xpress Micro and Mini Dialyzer

SERVA BlueNews 6/2016

New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

New SERVA Brochures:
All you need for ...

The SERVA Catalog 2015/16:

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Product of the Month September:

Xpress Micro and Mini Dialyzer

Ready-to-use devices for fast and efficient dialysis of proteins, DNA and RNA samples ranging from 10 to 1000 µl

This way to the campaign

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SERVA BlueNews 6/2016

Please find the latest information
on new products and services here:

• 1D SDS TA Gels
• EPO Doping Analytics
• DNADecon
• New Tools in Dialysis
• Product of the Month
• Western Blot Workflow
• Sample Preparation by Protein Ark
• LED Gel Scanner BIO-1000F
• Collagenase AF GMP Grade
• Top Ten YouTube Videos

BlueNews: Issue June 2016.pdf
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New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP
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The SERVA Catalog 2015/16:

• Biochemicals A-Z

• Sample Preparation

• Electrophoresis

• Laboratory Equipmeng

• Collagenase & Neutral Protease

• Life Sciences

• Enzymology

• Microscopy

• Ion Exchange Media

• Pharmacopoeia

Find the SERVA Online Catalog 2015/16 here!

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