Home › New Products Electrophoresis
Bio-5000 VIS Gel Scanner
Bio-5000 Professional VIS Gel Scanner for SDS PAGE gels,
Western Blots etc.
Microtek‘s Bio-5000, performing advanced application in biological laboratory, is perfectly designed for image capture of dry or wet samples of gel electrophoresis, SDS-PAGE, IEF gels, western blotting films, documents and so on. The Bio-5000, presenting greater image quality, has great features including 4800 optical resolution, 48-bit color scanning mode and linearity over 4.0 O.D.
- Designed removable waterproof glass holder
- Deliver the accurate images no matter the scanned target
is dry or wet gel samples - Make batch scanning easily
- Mechanism designed of Microtek patented E.D.I.T. technology
Specification:
| Scanning Modes | Color and grayscale, single scanning pass True 48-bit-color 16-bit grayscale (65,536 shades of gray) |
| Scanning Area | Reflective: max. 216 mm x 356 mm Transparency: max. 216 mm x 260 mm |
| Linearity | 4.0 O.D. |
| Resolution | 4800 dpi x 9600 dpi |
| Interface | Hi-Speed USB (USB 2.0) |
| Dimension | 567 mm x 385 mm x 158 mm (L x W x H) |
| Weight | 12 kg |
| Voltage | AC 100 V to 240 V; 50 - 60 Hz |
| Power | 30 W |
HPE - High Performance Electrophoresis
The new multi-level electrophoresis system for up to four high resolution
1D and 2D separations in flatdbed gels!
The HPE FlatTop Tower and the HPE gels have been developed together as a system, in order to achieve better results than can be obtained with conventional SDS polyacrylamide gel electrophoresis (PAGE) technology.
The Equipment: HPE Tower System
The Flatbed Gels: 2D SDS PAGE Gel Kits
The Flatbed Gels: 1D SDS PAGE Gel Kits
The Flatbed Gels: FocusGels for Isoelectric Focusing (IEF)
The Vertical Gels: Precast 2D Vertical Gel Kits
The HPE Tower System
The SERVA HPE tower consists of four horizontal electrophoresis chambers, which are built as movable drawers into a metal housing. The flatbed gels are placed on aluminium oxide ceramics cooling plates, which ensures very efficient heat dissipation and therefore straight electrophoresis migration in each gel. The HPE tower also has a built-in pump for optimum cooling water flow through the plates. When needed, electrophoresis buffer is supplied by fibre wicks, soaked with concentrated buffer. The electrode positions are adjustable to different gel sizes. The HPE tower runs with an external power supply (HPE-PSP) and a thermostatic circulator (cat. No. HPE-CU1), both included with the HPE tower system (HPE-TS1).
BlueLine Tower System - Products
The Flatbed Gels
To complete the HPE technology flatbed gels for different applications are available:
2D SDS PAGE Gel Kits
2D SDS Page gel kits are available in three different formats:
• HPE Large Format Gels: suitable for running 1 x 24 cm IPG Strip + marker lane
• HPE Double Gel Kits: suitable for running 2 x 11 cm IPG Strip + marker lane
• HPE Triple Gel Kits: suitable for running 3 x 7 cm IPG Strip + marker lane
All gels can be run by horizontal electrophoresis on HPE Tower and BH-2C. Gels are on standard backing for Silver or Coomassie staining or on non-fluorescence (NF) backing for best results with fluorescent staining and labeling. Each kit contains 4 gels + buffer kit (including running and equilibration buffer, buffer wicks and cooling contact fluid).
HPE Precast 2D Flatbed Gels - Products
1D SDS PAGE Gel Kits
There are different gel types and application optimized buffer kits available:
| • | 1D SDS PAGE gel kits are ready to use kits for SDS PAGE. The gel concentration of the gels are 10, 12.5 or 15 % (T) acrylamide. They are available with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. Each kit contains 4 gels + buffer kit. All gels can be run by horizontal electrophoresis on HPE Tower and BH-2C. |
| • | CleanGels are rehydratable gels are available with acrylamide content of 10 or 15 % (T) and with 25 (type 25S for 15 µl/slot) or with 52 (type 52S with 6 µl/slot) slots. Gels are on standard backing. A special formulated ampholyte mixture for CSF analysis on CleanGels is available. |
| • | Sortex-Kit for Cereal-cultivar-differentiation-electrophoresis. Contains 4 gels on standard backing and special buffer kit for flatbed electrophoresis on HPE tower and BH-2C. |
| • | Native Buffer Kit contains buffer system for native cathodal flatbed electrophoresis of proteins on HPE tower and BH-2C. |
| • | SDS Neutral Buffer Kit contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks for flatbed electrophoresis on HPE tower and BH-2C. |
| • | SDS Borate Buffer Kit for flatbed SDS PAGE of high molecular weight proteins on CleanGels. Contains anode buffer, cathode buffer, equilibration buffer, cooling contact fluid and electrode wicks. |
The Precast 2D Vertical Gel Kits
The 2D DALT gel kits contain 6 or 12 gels for ETTAN DALTsix and ETTAN DALTtwelve, respectively. They contain 6 or 12 gels + buffer kits containing anodal and cathodal buffers as well as IPG equilibration buffer and agarose for strip fixation.
Precast 2D Vertical Gel Kits - Products
Flatbed Gels for Isoelectric Focusing (IEF)
The FocusGels are rehydratable gels for flatbed isoelectric focusing on HPE Tower and BH-2C. They are available in 9 different pH ranges. The gels have none, 24 (type 24S with 25 µl/slot) or 40 (type 40S with 12 µl/slot) slots. Ampholyte mixture for rehydration and dilution buffer required for running IEF gels is available.
FocusGels - Products
SERVASnow Staining Kit
Rapid, sensitive and completely reversible protein staining method
The SERVASnow Staining Kit is a zinc-based protein gel stain as sensitive as other commonly used protein staining techniques, but with the great advantage of being erasable. The simple staining protocol results in a stained gel with an opaque milky-white background and clear protein bands. The white background is easily removed by incubation in a mobilizing buffer. The gel can then be restained by any other method or the proteins can be transferred to a membrane for Western Blotting or eluted from an excised gel piece.bands. The white background is easily removed by incubation in a mobilizing buffer. The gel can then be restained by any other method or the proteins can be transferred to a membrane for Western Blotting or eluted from an excised gel piece.
35080_SERVA SnowPR 250 Mini Orbital Shaker
The Digital LED display and easy programming sequence makes setting speed and time simple; the timer can be set from 0 to 99 hours 59 minutes or set to run continuously. The PR250 Mini Orbital Shaker speed can be adjusted to from 50 to 250 rpm, and has an orbit of
20 mm. The unit has a small footprint and is cold room compatible for applications that require refrigeration.
Key Features:
• Variable speed 50 to 250 rpm
• Cold room compatible
• Flat platform with skid resistant mat
• Orbit of 20 mm Quiet sealed bearing rotation
• LED Display
• 99 hour 59 minutes timer with audible end of run alert
• Capable of continuous run
• Small footprint
• Universal voltage
• Optional accessory platforms available
• CE marked
There are three accessory tables available separately which can be easily installed:
a twelve place 100 ml beaker clamping table, a six place 250 ml beaker clamping table and a universal table with two adjustable bar clamps.
Each model includes: shaker, flat platform with skid resistant mat, screw driver.
Agarose SERVA Wide Range
molecular biology grade
Agarose SERVA Wide Range (molecular biology grade) is ideal for rapid separation of nucleic acids from 250 bp to 23,000 bp. It is easily dissoluted in electrophoresis buffer and features a very low DNA binding. Excellent transparency and low background staining result in very clear band visibility. Suitable for blotting experiments as well as other common molecular biology techniques as preparation of plasmids, screening and cloning. DNase/RNase-free.
11406 Agarose SERVA Wide Range
SERVA DNA Stain G
Non-carcinogenic alternative
to Ethidium Bromide
SERVA DNA Stain G is a safer alternative to traditional ethidium bromide stain for detecting nucleic acid in agarose gels. It is at least as sensitive as ethidium bromide and can be used in exactly the same way in agarose gel electrophoresis. SERVA DNA Stain G can be added to the gel solution, working dilution is 1:20.000 to 1:50.000.
39803 SERVA DNA Stain G
SERVAGel™ N
Vertical precast gels for native PAGE
• Suitable for both Blue and Clear Native PAGE
• Ready-to-use precast gels
• Available as gradient gels: 3-12 % and 4-16 %
• Available with 10 (max. sample volume 50 µL) or 12 (max. sample volume 35 µL) sample wells
• Produced in stable plastic cassettes (10 cm x 10 cm)
SERVAGel™ Precast gels - Overview
SERVAGel™ IEF
The new SERVAGel ™ IEF:
- For vertical IEF in mini slab gel format
- In unbreakable plastic cassette (10 x 10 cm)
- Available in pH range 3 – 10
- Compatible with standard vertical mini slab gel apparatus, no special equipment needed
- Suitable for both native and denaturing conditions
- Available as SERVAGel ™ IEF Starter Kit
- Also available: SERVAGel ™ IEF Runing Buffer Kit
SERVAGel™ Precast gels - Overview
SERVA IPG BlueStrips
Broadest range of IPG strips now available from SERVA::
- Strip lengths available: 7, 11, 17, 18 and 24 cm
- pH gradients available: 3-10, 3-10 NL, 3-6, 4-7,
5-8, 6-10 - For rehydration in convenient 2ml pack sizes: SERVALYT™ Carrier Ampholytes
3-10, 3-10 IsoDalt, 3-6, 4-7, 5-8 and 6-9 - All gel strips are 3 mm wide
- Other pH gradients and gel strip lengths are available upon request
New Product: SERVA IPG BlueStrips
SERVA IPG BlueStrips - Overview
SERVA BluePower™ Power Supplies
Now available with 4 x 2 outlets
Different power supplies are needed to cover the large variety of separations such as SDS and native PAGE electrophoresis of proteins, DNA electrophoresis in agarose and PAGE gels, Isoelectric Focusing, 2D electrophoresis and electrotransfer of biomolecules e.g. Southern-, Northern- and Western-Blotting.
All SERVA BluePower power supplies are easy-to-use, safe and reliable. They are fully overload-protected including short-circuit of outputs: an automatic poweroff function stops the voltage when ground leakage is detected.Now all SERVA BluePower™ power supplies are equipped with 4 x 2 outlets, 4 electrophoresis units can be operated in parallel.
BluePower™ Power Supplies
SERVA ICPL™ Quadruplex Kit
Now with a fourth label!
The powerful ICPL™-technology for comparative quantification of proteins is now available with a fourth label. Applying the ICPL™ Quadruplex method the simultaneous quantitative analysis of four independent proteome samples can be performed by stable isotope protein labelling of all lysine residues and protein N-termini. The ICPL™-technology combines the power of stable isotope coded labelling with the concept of reducing the proteome complexity by fractionation of intact proteins.
Data evaluation with ICPLQuant – freeware and easy to use
A new software - ICPLQuant – was developed for analysis of LC/MS data of ICPL™ – labelled proteins (Brunner et. al., ICPLQuant – A software for non-isobaric isotope labelling proteomics, Proteomics 10, 2, (2010)). The software tool is free of charge and can be downloaded from here.
The main functionalities are peptide pattern searching (ICPL duplex, triplex, quadruplex), peptide quantification in an LC-run and merging with MASCOT protein results. ICPLQuant takes deisotoped data as input, generates precursor files for MS/MS and is able to import MASCOT result files for combining MS/MS and quantification results.
By selecting only regulated precursors for subsequent MS/MS experiments, the software minimizes time consuming acquisition and interpretation of MS/MS data. Up to now, ICPLQuant is able to handle data generated by MALDI (Applied Biosystems 4700/4800, Bruker Ultraflex) and ESI (Thermo Orbitrap) machines.
SERVA BluePrep - Kits for protein and protein/nucleic acid isolation and purification
Reliable, efficient and easy sample preparation is a crucial step for achieving optimal and reproducible results in proteomics. The SERVA BluePrep Kits cover sample preparation steps from protein isolation, concentration, buffer exchange, removal of interfering substances, tryptic digestion of proteins to abundant serum protein depletion or recombinant protein extraction from E. coli.
The produced samples can be used directly in applications such as SDS-, 2D or native PAGE, microarrays, immunoblotting, mass spectrometry, and others.
SERVA BluePrep - Kits for DNA/RNA/protein sample preparation
For studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery, researchers are interested in a comparative analysis of the genome, transcriptome and proteome of a sample. So far one sample had to be splitted for isolation of DNA, RNA and proteins.
The novel SERVA BluePrep 2in1, 3in1 and 4in1 Kits allow the sequential purification of DNA, RNA and proteins from a single sample using one column without sample splitting. The kits offer substantial cost and time savings and eliminate the variability and inconsistence of results associated with sample splitting.
BlueMarine HTS
The new submarine electrophoresis chamber BlueMarine HTS offers you an innovative, flexible system for DNA analysis. If you need high resolution long runs of single samples or analysis of complete 96-well microtiter plates, the BlueMarine HTS allows for a fast and clear analysis of up to 102 samples. By changing the left/right orientation of the comb you can position the sample wells one upon the other or shifted to each other. The system contains the submarine chamber and one casting tray (UV-transparent) plus two casting gates and six combs (17 sample wells, 1 mm) made from stable and indestructible aluminum.
BlueMarine™ HTSSingleQuant
Precise, reliable and simple determination of protein concentration in single tube format.
more
DIAS-II: The new Gel Documentation and
Analysis System from SERVA
The totally new designed Digital Imaging and Analysis System II (cat. no. DIAS-II) allows versatile applications in documentation and analysis. DNA- or protein gels, EtBr-, Coomassie™ or silver staining, thin layer chromatography or bacterial culture plates, the new DIAS II serves all requirements you expect from a state of the art gel documentation and analysis system.
The DIAS-II system consists of the following components:
- a light-tight and light-weight cabinet (ca. 40 x 50 x 40 cm)
- a digital 12.1 MP digital camera (Canon PowerShot G9)
- a powerful 1D analysis software (GelScan 6.0)
The DIAS-II system can be combined optionally with:
- an UV transilluminator (cat. no. ECX-20.M, 312 nm, dual intensity
at 100 % and 70 %) - an UV/WL conversion plate (cat. no. FC-26.WL, in combination
with ECX.20-M, only) - an UV-/WitheLight-Top Light (cat. no. 14-EL-254 [254 nm/WL] or 14-EL-312
[365 nm/WL) - UV/Blue Light (480 mnm) Conversion Screen for ECX-20.M (cat. no. FC-26.BL);
for GFPII, Syber Green or Syber Orange - a white light table
- a PC to operate the system
- a thermoprinter for direct print out of images
GelScan 6.0 1 D Analysis Software
New 2-Dimensional Electrophoresis System by Hoefer
Hoefer introduces a complete 2-Dimensional Electrophoresis System that offers the highest focusing voltage available for the first dimension and has an easy-to-use, highly reproducible, second-dimension separation tank.
The system includes:
The IEF100 First-dimension Isoelectric Focusing Unit
The IEF100 integrated 12 000 Volt Power Supply enables fast run times. Up to six IPG strips of any length commercially available (e.g. SERVA IPG BlueStrips, see below) can be focused at one time and monitored individually.
SERVA IPG BlueStrips - OverviewThe SE900 Separation Tank
The SE900 separation tank can hold up to 6 second dimension gels. It is simple to use, utilizes minimal parts, and provides efficient cooling. The multiple gel caster that can be purchased with the SE900, allows casting of identical gels leading to highly reproducible production of self cast gels.
2D Electrophoresis System
SERVAGel™ - The New Precast Vertical 2D-Gel!
SERVA offers precast gels in cassettes for two-dimensional electrophoresis of proteins in mini gel format.
more
New Power Supply Line from Hoefer
Hoefer introduces stylish new Power Supplies, PS300B and PS200HC.
more
