SERVAGel™ precast vertical gels for mini slab gel electrophoresis
are ready-to-use, polyacrylamide gels cast into unbreakable plastic cassettes.
The gels are individually packed in a vacuum sealed bag. Using the precast
gels you can separate your protein samples in the presence (SDS PAGE) or
absence (native PAGE) of SDS. The main benefits of SERVAGel™ precast
gels are the superior separation performance, the excellent
staining/destaining properties and the overall easy handling. With the 1 mm
thin gels 10, 12 or 15 samples can be analyzed, the separation distance is 7
cm. The cassette format of 10 x 10 x 0.7 cm fits perfectly into SERVA's
innovative BlueVertical™ PRIME™ Mini Slab gel Unit. However, the cassette gels
are as well compatible with most commercially available slab gel tanks, e. g.
the mini vertical systems Mighty Small II (SE 260) and miniVE (SE 300) from
superb band sharpness
Easy and safe to operate, no leakage
Short set-up times, gels are ready-to-use
Unbreakable plastic cassette,
Risk to health reduced to minimum (polymerized acrylamide, no
SERVAGel™ Vertical Gels for 1D- and 2D-SDS
PAGE available as
Gel™ TG PRiME™ Tris/Glycine gel
for standard and fast electrophoresis (approx. 35 min) according to Laemmli
(Nature, 1970) with long shelf life
Gel™ Neutral gel pH
7.4 with long shelf life, suitable for different running buffer, e.g.
Tris/Glycine or Tris/Tricine, and a separation range from 5 up to 200 kDa
Gel™ Neutral HSE gel for high speed electrophoresis (20 min) with
long shelf life
The SERVAGel™ N gels (cat. nos.
43250, 43251, 43252, 43253) were developed for native gel electrophoresis.
These gels can be operated in Blue and Clear Native buffer systems. Due to
their neutral buffer system, the SERVAGel™ N gels feature extended
shelf life. SERVAGel™ is a trademark of SERVA. PRiME™ stands
for Premium Resolution in Minigel Electrophoresis.
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ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.
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