Collagenase & Neutral Protease
Collagenases – For Tissue Dissociation
Collagenases from Clostridium histolyticum are proteolytic enzymes that
are able to cleave peptide bonds in the triple helical collagen molecule of
human or animal tissue in situ.
The collagenases are divided into class I and class II collagenase isoforms on the basis of their activities towards synthetic peptides. Both collagenase isoforms act on triple helical type I, II, III and IV collagens, but in slightly different modes of action.
SERVA Collagenase NB grades are particularly suitable for various
tissue isolation applications. The application of pharmaceutical manufacturing
standards guarantees stringent quality control, high quality, reliable
lot-to-lot consistency and excellent performance of the product.
Information about TSE safety is stated on certificates of origin for each product.
Reference Collagenases N (cat. no. 17463, 17464) are available for
enzyme activity determination or incoming goods inspection according to cGMP.
Anti-Collagenase polyclonal antibody from sheep (cat. no. 58050) and
Anti-Collagenase HRPpolyclonal antibody from sheep (cat. no. 58051) can be
used in ELISA and Western blot experiments, immunostaining of tissue and
analysis of residual collagenase impurities in cell preparations.
Stability and Storage
Collagenase requires calcium ions both for full catalytic activity and binding
to the collagen molecule. Collagenase NB grades are soluble in water and
common buffers. The enzyme is reversibly inactivated at high pH values and
irreversibly inactivated at low pH values.
Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. It is also inhibited by TRIS buffer above pH 7.5. Collagenase is not inhibited by diisopropylphosphorofluoridate.
All Collagenase NB products delivered as lyophilized powders can be stored at +2 to +8 °C. Lyophilized enzymes should be protected from moisture.
Dissolved enzymes can be aliquoted and stored at -20°C for one year. Repeated freezing and thawing should be avoided.
Conversion rates of collagenase activity units
SERVA states collagenase activity in PZ units according to Wünsch:
1 PZ U/mg ~ 3.9 FALGPA U/mg
1 PZ U/mg ~ 1000 Mandl or CDU U/mg
1 PZ U/mg ~ 10 HP U/mg