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HomeCollagenase & Neutral Protease

Collagenase & Neutral Protease

Collagenases – For Tissue Dissociation

Collagenases from Clostridium histolyticum are proteolytic enzymes that are able to cleave peptide bonds in the triple helical collagen molecule of human or animal tissue in situ.
The collagenases are divided into class I and class II collagenase isoforms on the basis of their activities towards synthetic peptides. Both collagenase isoforms act on triple helical type I, II, III and IV collagens, but in slightly different modes of action.

SERVA Collagenase NB grades are particularly suitable for various tissue isolation applications. The application of pharmaceutical manufacturing standards guarantees stringent quality control, high quality, reliable lot-to-lot consistency and excellent performance of the product.
Information about TSE safety is stated on certificates of origin for each product.

SERVA Collagenase AF grades
Animal Free (AF) collagenases are our superior enzymes designed to provide the highest possible safety for clinical applications. A plant-based production process and the use of carefully selected materials ensure that there is no commingling with components of animal origin. Thus, any risk of introducing potential animal-derived pathogen is avoided in the first place.

Reference Collagenases N (cat. no. 17463, 17464) are available for enzyme activity determination or incoming goods inspection according to GMP.

Anti-Collagenase polyclonal antibody from sheep (cat. no. 58050) and Anti-Collagenase HRP polyclonal antibody from sheep (cat. no. 58051) can be used in ELISA and Western blot experiments, immunostaining of tissue and analysis of residual collagenase impurities in cell preparations.

Stability and Storage

Collagenase requires calcium ions both for full catalytic activity and binding to the collagen molecule. Collagenase NB grades are soluble in water and common buffers. The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Collagenase activity is at an optimum at 37 °C and pH 7.4.
Inhibitors of collagenase include cysteine, EDTA,
o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. Collagenase is not inhibited by diisopropylphosphorofluoridate.
All Collagenase NB products delivered as lyophilized powders can be stored at +2 to +8 °C. Lyophilized enzymes should be protected from moisture.
Dissolved enzymes can be aliquoted and stored at -20 °C for one year. Repeated freezing and thawing should be avoided.

Conversion rates of collagenase activity units

SERVA states collagenase activity in PZ units according to Wünsch:
1 PZ U/mg ~ 3.9 FALGPA U/mg
1 PZ U/mg ~ 1000 Mandl or CDU/mg
1 PZ U/mg ~ 10 HP U/mg

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ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP

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