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HomeCollagenase & Neutral Protease

Collagenase & Neutral Protease

Collagenases – For Tissue Dissociation

Collagenases from Clostridium histolyticum are proteolytic enzymes that are able to cleave peptide bonds in the triple helical collagen molecule of human or animal tissue in situ.
The collagenases are divided into class I and class II collagenase isoforms on the basis of their activities towards synthetic peptides. Both collagenase isoforms act on triple helical type I, II, III and IV collagens, but in slightly different modes of action.

SERVA Collagenase NB grades are particularly suitable for various tissue isolation applications. The application of pharmaceutical manufacturing standards guarantees stringent quality control, high quality, reliable lot-to-lot consistency and excellent performance of the product.
Information about TSE safety is stated on certificates of origin for each product.

Reference Collagenases N (cat. no. 17463, 17464) are available for enzyme activity determination or incoming goods inspection according to GMP.

Anti-Collagenase polyclonal antibody from sheep (cat. no. 58050) and Anti-Collagenase HRP polyclonal antibody from sheep (cat. no. 58051) can be used in ELISA and Western blot experiments, immunostaining of tissue and analysis of residual collagenase impurities in cell preparations.

Stability and Storage

Collagenase requires calcium ions both for full catalytic activity and binding to the collagen molecule. Collagenase NB grades are soluble in water and common buffers. The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values.
Inhibitors of collagenase include cysteine, EDTA,
o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. It is also inhibited by TRIS buffer above pH 7.5. Collagenase is not inhibited by diisopropylphosphorofluoridate.
All Collagenase NB products delivered as lyophilized powders can be stored at +2 to +8 °C. Lyophilized enzymes should be protected from moisture.
Dissolved enzymes can be aliquoted and stored at -20 °C for one year. Repeated freezing and thawing should be avoided.

Conversion rates of collagenase activity units

SERVA states collagenase activity in PZ units according to Wünsch:
1 PZ U/mg ~ 3.9 FALGPA U/mg
1 PZ U/mg ~ 1000 Mandl or CDU/mg
1 PZ U/mg ~ 10 HP U/mg




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• Biochemicals A-Z

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New Application Note:
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ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.


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