Isoelectric Focusing (IEF) can be described as an ingenious process for
simultaneous concentration and separation of proteins. IEF employs a pH
gradient formed by small amphoteric molecules (called ampholytes) to resolve
proteins according to their different pI values (pI = isoelectric point). IEF
is an end point method - when the electrophoretic run is completed proteins
will appear as separate, sharp zones, in the order of their isoelectric points.
For IEF, SERVA offers a comprehensive product line: