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Isoelectric Focusing

Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.

For IEF, SERVA offers a comprehensive product line:

  • SERVALYT™ Carrier Ampholytes
  • SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
  • Protein Standards
  • Buffers and Accessories
  • Equipment


    • Saturday, February 11, 2012

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