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HomeElectrophoresisIsoelectric Focusing

Isoelectric Focusing

Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.

For IEF, SERVA offers a comprehensive product line:

  • SERVALYT™ Carrier Ampholytes
  • SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
  • FocusGels Precast Horizontal Gels
  • SERVA
  • Gel™ IEF Vertical Gels
  • Protein Standards
  • Buffers and Accessories
  • Equipment


  • Wednesday, October 1, 2014

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    Große Herbstaktion -
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    New Application Note:
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    SERVA Promotions IX/2014



    Produkt des Monats September
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    Verleihung des Transferpreises 2013/2014
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    an Prof. Dr. Simone König und SERVA Electrophoresis GmbH

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    New Brochure:
    Instruments for Life Sciences Research!


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    Große Herbstaktion -
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    • Biochemicals A-Z

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    Find the SERVA Online Catalog 2014 here!

    New Application Note:
    ICPL™ - Coupled Immunoprecipitation
    (ICPL-IP)

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    ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.


    SERVA ICPL™ Quadruplex Plus Kit 39233
    Application Note: ICPL-IP

    SERVA Promotions IX/2014

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    Verleihung des Transferpreises 2013/2014
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    an Prof. Dr. Simone König und SERVA Electrophoresis GmbH

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    Die Chemikerin Prof. Dr. Simone König erhielt am 3. Juni 2014 den
    mit 20.000 € dotierten Transferpreis der Westfälischen Wilhelms-Univer-
    sität (WWU) für die Verbesserung einer in der Labortechnik sehr häufig eingesetzten Methode der
    Gel-Elektrophorese.

    Geehrt wurde außerdem Günter Theßeling von der Firma SERVA Electrophoresis GmbH in Heidelberg, die die Entwicklung jetzt auf den Markt bringt.

    Film: Von der Idee zum Mercator-Gel


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