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Hoefer Inc., USA

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IMPLEN GmbH, D

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Isogen Life Science B.V.

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Generon Ltd., UK

GST-tagged Protein Purification

Pro-Chem Inc., USA

Antibody Purification

 

HomeElectrophoresisIsoelectric Focusing

Isoelectric Focusing

Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.

For IEF, SERVA offers a comprehensive product line:

  • SERVALYT™ Carrier Ampholytes
  • SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
  • FocusGels Precast Horizontal Gels
  • SERVA
  • Gel™ IEF Vertical Gels
  • Protein Standards
  • Buffers and Accessories
  • Equipment


  • Thursday, April 24, 2014

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    New Application Note:
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    ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.


    SERVA ICPL™ Quadruplex Plus Kit 39233
    Application Note: ICPL-IP

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