Enzymes in Mass Spectrometry

Trypsin, MS approved is especially designed for digestion of proteins prior to mass spectrometry analysis. Every lot is approved for use in in-gel digestion and mass spectrometry analysis. Reductive methylation of the lysine residues of trypsin results in a stable product that is extremely resistant to autolytic degradation. Trypsin, MS approved is purified by chromatography. No chymotryptic activity is detectable.

Endoproteinase Glu-C, recombinant (V8) is a serine endoproteinase used with in-gel digestion and mass spectrometric analysis. The specificity of Glu-C is primarily determined by the buffer pH and composition. Using phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds. Ammonium bicarbonate buffer (pH 7.8) will lead to a preferential cleavage of glutamyl bonds. The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage. Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work.

Lysyl Endopeptidase® is especially designed for use with in-gel digestion and mass spectrometric analysis. Lysyl Endopeptidase (LysC), originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for proteome research. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4 M urea or in 0.1 % SDS solution for up to 6 hours at 30 °C.

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SERVA Catalog
2023

Electrophoresis by SERVA - Catalog

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SERVA Catalog
2023

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
SERVA Catalog 2023.pdf
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Electrophoresis by SERVA - Catalog

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
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Certificates of Analysis
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How to find the required certificate of analysis for your product:
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Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

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