Enzymes in Mass Spectrometry

Trypsin MS approved is suitable for digestion of proteins for mass spectrometry analysis. Reductive methylation of the lysine residues of trypsin results in a stable product that is extremely resistant to autolytic degradation. Trypsin MS approved is purified by chromatography. No chymotryptic activity is detectable. Every lot is approved for use in in-gel digestion and mass spectrometry analysis. Specificity verified by digestion of oxidized B-chain of insulin. A Trypsin Peptide (TP) Standard is available separately (cat. no. 37285) allowing internal calibration for enhancing mass accuracy. The TP standard provides tryptic activity to generate masses m/z 842 and 2211.

Endoproteinase Glu-C (V8) is a serine endoproteinase isolated from Staphylococcus aureus V8. Approved quality for use with in-gel digestion and mass spectrometric analysis. The specificity of Glu-C is primarily determined by the buffer pH and composition. Using phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds. Ammonium bicarbonate buffer (pH 7.8) will lead to a preferential cleavage of glutamyl bonds. The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage. Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work.

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ICPL™ - Coupled Immunoprecipitation
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Product of the Month April:

Ni-NTA Magnetic Agarose Beads

Purification of polyHis-Tagged Recombinant Proteins

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The SERVA Catalog 2017/18:

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SERVA BlueNews 6/2016

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New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP
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