Classification of Detergents

Surfactants can be classified according to their charge, as follows:

Nonionic detergents contain uncharged hydrophilic head groups that consist of either polyoxyethylene moieties as in BRIJ or TRITON, PEG-sorbitan units as in TWEEN, or glycosidic groups as in octyl-ß-D-glucoside, dodecyl-ß-D-maltoside or digitonin. They are well suited for breaking lipid-lipid and lipid-protein interactions but normally do not break protein-protein interactions. Compared to ionic detergents, they usually do not denature proteins and are therefore frequently used for the isolation of biologically active membrane proteins.

Ionic detergents contain a head group which is either positively or negatively charged. For example, the anionic detergent sodium dodecyl sulfate (SDS) carries a negatively charged sulfate group on a linear C12 hydrocarbon chain. SDS is considered as a very strong and biologically harsh surfactant. It is able to denature proteins by breaking intra- and intermolecular interactions and thus destroying their biological activity. Other anionic detergents like the bile acid salts Na-cholate and Na-deoxycholate have a rigid steroidal core structure. They do not carry a well defined polar head group opposite to a hydrophobic tail (such as SDS). The polar groups are distributed on different parts of the molecule, resulting in a polar and non-polar side, e.g. Na-deoxycholate carries a carboxylate group at the end of a short hydrocarbon chain and two hydroxyl groups on the steroid structure. The bile acids are less denaturing than the ionic alkyl detergents, possibly due to their rigid steroidal ring structure.

Cationic detergents have a positively charged head group, which is often a quaternary ammonium group, e.g. CTAB carries a trimethylammonium group on a C16 hydrocarbon chain. It is also a strong detergent and will often irreversibly denature proteins.

Zwitterionic detergents like CHAPS or SB12 combine the properties of ionic and nonionic detergents. They carry a positively and a negatively charged group, but like nonionic surfactants, do not have a net charge (for CHAPS and sulfobetaines in the pH range of 2 -12). Furthermore they lack conductivity and electrophoretic mobility and do not bind to ion exchange resins. However, they are able - like ionic detergents - to break protein-protein interactions.

Detergents - Overview

Cloud Point

 Nonionic Detergents     

 Brij 35
 ca. 1200
 0.67 - 0.73
 Lubrol 17A17
 Synperionic F68
 ca. 8300
 Synperonic F108
 ca. 14000
 Triton X-100
 ca- 625
 63-69 (1 % in H2O)
 Tween 20
 76 (3 % in 1 N NaCl solution)
 Tween 80
 65 (3 % in 1 N NaCl solution

Ionic Detergents

 7 - 16
 2 - 7
 2.4 - 4
 2 - 19.9
 Na-Dioctyl sulfosuccinate

Zwitterionic Detergents

 4.2 - 6.5
 9 - 10
 SB 3 - 10
 25 - 40
 SB 3 - 12
 3 6



Product of the Month October:
Trypsin 1:250
for Cell Culture

Autumn Campaign 2017 -
Great Saving!

Hoefer Promotion -
valid until 31.12.2017

BioDrop Promotion -
valid until 31.12.2017

Close window
Product of the Month October:
Trypsin 1:250
for Cell Culture

For gentle dissociation and passaging of monolayers with maximum cell viability.

This way to the Special Offer

Close window
Autumn Campaign 2017 -
Great Saving!
  • PRiME™-Packages for Minigel SDS PAGE
  • Protein Electrophoresis
  • Nucleic Acid Electrophoresis
  • Western Blotting
  • Laboratory Equipment
  • Trypsin MS Approved
  • DNA Decon

This way to the campaign

Close window
Hoefer Promotion -
valid until 31.12.2017

Save 25 % :

  • Mini Vertical Electrophoresis Units
  • Standard Vertical Electrophoresis Units
  • Tank Blotter

This way to the Promotion

Close window
BioDrop Promotion -
valid until 31.12.2017

BioDrop UV/VIS Spectrophotometer at a Glance:

  • BioDrop TOUCH
  • BioDrop µLITE
  • BioDrop DUO

This way to the promotion!


SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.