II. SERDOLIT® PAD Adsorber Resins

Whereas ion exchangers (mainly) bind charged molecules, neutral adsorbent resins can be used for the extraction and in some cases even for the separation of neutral, basic and acidic substances. This adsorption process often is performed with compounds which are dissolved in water or in a solution which does not contain too much of an organic solvent that might prohibit the interaction (van der Waals forces, hydrophobic, hydrophilic bridges) between adsorbent and adsorbate.
SERVA offers 2 types of adsorber resins: polar and unpolar ones. The unpolar types SERDOLIT®  PAD I, II and III are macroporous copolymerisates of polystyrene and DVB. They are supplied each in 2 particle sizes: 0.1 – 0.2 mm and 0.3 – 1.0 mm. They have similar pore sizes (approx. 25 nm) but differ in specific surface:

 PAD I  min. 250 m2/g
 PAD II  min. 400 m2/g
 PAD III  min. 800 m2/g

Generally speaking, the larger the surface, the higher is the capacity.

The polar resin SERDOLIT® PAD IV is a macroporous copolymerisate of acrylic ester and DVB. It is supplied with a particle size of 0.3 – 1.0 mm and has a pore size of approx. 20 nm and a specific surface of min. 250 m2/g.

Unpolar resins bind organics from water, polar resins even from organic solvents. Unwanted substances (impurities, salts) are eluted with water, the enriched compound(s) with water mixable organic solvents. Their eluotropic forces are known from chromatographic experience. Acidic molecules are best eluted with basic eluents, basic molecules under acidic conditions.
SERVA´s PAD resins are highly purified adsorbents which are prewetted (hydrophilized) and can be used straight away. These fine mesh resins may be used not only for solid phase extraction (SPE) but also in the well known reversed phase low and medium pressure chromatography up to 10 bars (140 psi) with the advantage to be hydrolytically stable within the entire pH range.
Table 9 lists compounds which can be extracted from water resp. waste water with SERDOLIT® PAD resins.

Table 9: Selection of compounds adsorbed with SERDOLIT® PAD resins

Aniline & substituted anilines
Benzene & substituted benzenes
Benzoic acid & its derivatives
Chlorinated hydrocarbons                     
Fatty acids
Humic acids and humic-like substances
Organophosphorous compounds
Organosulfur compounds
Phenol & derivatives
Polychlorinated biphenyls & other chlorinated compounds
Polynuclear aromatic compounds
Purine & pyrimidine bases and derivatives
Pyridine & substituted pyridines


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SERVA BlueNews 6/2016

New Application Note:
ICPL™ - Coupled Immunoprecipitation

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The SERVA Catalog 2017/18:


• Biochemicals A-Z

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SERVA BlueNews 6/2016

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BlueNews: Issue June 2016.pdf
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New Application Note:
ICPL™ - Coupled Immunoprecipitation

ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP
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