Sample Preparation

SERVA Ni-NTA Magnetic Beads

Nickel-NTA magnetic agarose beads for purification of His-tagged proteins

  • Rapid and easy purification under native or denaturing conditions
  • Ideal for small sample volumes
  • High binding capacity: > 75 mg His-tagged protein/ml gel
  • No need for protocol change – easy changeover from other resins

Xpress Micro Dialyzer

  • Highly innovative ready-to-use dialysis devices for small sample volumes
    (MD100: 10 µl – 100 µl,
    MD300: 50 µl – 300 µl)
  • High-quality and low-binding regenerated cellulose membranes
  • Efficient and fast dialysis – desalting of nucleic acids and proteins in ca. 30 min
  • High yield - up to 98 % sample recovery
Xpress-micro.jpg
  • Easy handling – sample loading and retrieval can be done with standard laboratory micropipettes
  • Flexible – scalable from 1 to 96 samples, dialysis in micro tubes/microtiter plates or in beakers with floating device possible
  • Automation compatible – plate format conforms to the SBS Microplate Standard

Xpress Mini Dialyzer

  • Highly innovative ready-to-use dialysis devices for sample volumes of 150 µl – 1000 µl
  • High-quality and low-binding regenerated cellulose membranes
  • Efficient and fast dialysis
  • High yield - up to 98 % sample recovery
Xpress-mini.jpg
  • Easy handling – sample loading and retrieval can be done with standard laboratory micropipettes
  • One step protocol – no laborious purification steps,
    no need for expensive lab equipment
  • Flexible – scalable from 1 to 48 samples
  • Automation compatible – plate format conforms to the SBS Microplate Standard

ReadyLyzer

  • Ready-to-use dialysis system for quick and efficient dialysis and buffer exchange
  • Easily opened and closed with a screw cap
  • No struggling with closures and leaking knots or risking puncturing the membrane
  • Delivered with a flotation ring for improved buoyancy and vertical orientation
ReadyLyzer.jpg
  • Ultrapure regenerated cellulose membrane
  • Available volume sizes: 10 - 250 µl, 50 – 800 µl, 0.1 – 3 ml, 10 ml, 20 ml
  • High recovery 98 %

SERVA Streptavidin Agarose Resin

Highly selective purification of biotinylated biomolecules

  • High specific activity recombinant streptavidin immobilized on highly crosslinked agarose
  • Very high binding capacity:
    >330 nmol biotin/ml gel
  • Low non-specific binding, less leaching
  • Suitable for batch and column purification
  • Empty columns for small and larger volumes available
Diagramm Streptavidin web.jpg

Glutathione Agarose Resin

Rapid, mild and highly selective purification of GST fusion proteins in an one step procedure

  • Fast and easy - high purity achieved with one purification step
  • Suitable for small and large protein complexes
  • Easy changeover from other resins: no need for optimization or
    protocol change
Agarose-Resins.jpg
  • Great adaptability – bulk format suitable for batch and column purification
  • Made of agarose beads – excellent chemical and mechanical stability
  • Empty columns for small and larger volumes available
    (50 – 100 µl/200 – 250 µl/0.5 – 2 ml/2 – 6 ml)

SERVA Ni-NTA Agarose Resin

Rapid and highly selective one step purification of His-Tag fusion proteins

  • High-protein binding and minimal metal leaching
  • Purification under native and denaturing conditions
  • No need for protocol change  – easy changeover from other resins
  • Suitable for batch and column purification
  • Empty columns for small and larger volumes

SERVA IDA Agarose Resins

Choose between four different types of metal chelates and two different densities of groups on the beads

  • Ni2+-, Co2+-, Zn2+- or Cu2+- charged as well as metal-free resins available – a solution for every purification problem
  • Two activation grades (Low Density and High Density) for optimizing the relationship between binding capacity and purification selectivity
IMAC-Agarose_1.jpg
  • Made of agarose beads – excellent chemical and mechanical stability
  • Broad range of test kits available for easy screening for the best option
  • Ready-to-use pre-packed columns for fast and simple purification
  • Cost-effective bulk resins suitable for batch and column purification
  • Empty columns for small and larger volumes available
    (50 – 100 µl/200 – 250 µl/0.5 – 2 ml/2 – 6 ml)

Gelfiltration Columns from emp Biotech – fast and efficient protein purification

CentriPure, CentriPure Mini Spin and CentriSpin columns – ready-to-use columns for fast and efficient desalting, buffer exchange and removal of small molecular impurities from protein samples.

emp_kit.jpg

The gel matrix Zetadex, a new cross-linked composite dextran matrix, has been proven to be superior for desalting, buffer exchange and removal of small molecular impurities, such as salts, ammonia, dyes, biotin, haptens etc. from antibodies, enzymes and other proteins.
• Hydrated, ready-to-use gel filtration columns
• Processing of small and large sample volumes possible
• Available as flow gravity or spin columns
• Fast and easy handling
• High yield and high purity
• Minimal effect of buffer and pH on resolution
• Purified biomolecules are directly suited for downstream applications, like SDS PAGE, mass spectrometry, X-ray crystallisation

Promotions

SERVA Market Square:

February - March 2017

Product of the Month March:

BlueBlock PF

The SERVA Catalog 2017/18:

online!

SERVA BlueNews 6/2016

New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

New SERVA Brochures:
All you need for ...

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SERVA Market Square:

February - March 2017

Save up to 30 % :

  • Antibiotics
  • Buffers and Buffer Solutions
  • Detergents
  • Staining Reagents
  • Western Blotting

This way to the Market Square

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Product of the Month March:

BlueBlock PF

Protein-free Blocking Reagent for Western Blots

This way to the Special Offer

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The SERVA Catalog 2017/18:

online!

• Biochemicals A-Z

• Sample Preparation

• Electrophoresis

• Laboratory Equipmeng

• Collagenase & Neutral Protease

• Life Sciences

• Enzymology

• Microscopy

• Ion Exchange Media

• Pharmacopoeia

Find the SERVA Online Catalog 2017/18 here!
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SERVA BlueNews 6/2016

Please find the latest information
on new products and services here:

• 1D SDS TA Gels
• EPO Doping Analytics
• DNADecon
• New Tools in Dialysis
• Product of the Month
• Western Blot Workflow
• Sample Preparation by Protein Ark
• LED Gel Scanner BIO-1000F
• Collagenase AF GMP Grade
• Top Ten YouTube Videos

BlueNews: Issue June 2016.pdf
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New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP
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