Salt Active Nucleasesolution

The Salt Active Nuclease completely degrades DNA and RNA in cell extracts and protein samples under high salt conditions. In the range of the activity optimum at 0.5 M NaCl, nucleic acids bound to proteins and protein complexes dissociate, whereby the endonuclease isolated from a marine bacterium also degrades DNA and RNA molecules that are inaccessible under low-salt conditions. The reaction takes place at a pH between 7 and 10 at low temperatures and in traditional buffer systems to protect the proteins.

  • Non-specific endonuclease
  • Optimum activity at high salt concentration (0.5 M NaCl)
  • Active at low temperatures (20 % at 6 ºC)
  • Broad pH range
  • Easily inactivated by reducing agents

  • Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl2, 0.5 M NaCl, 0.01 % Tween 20, 50 % (v/v) glycerol.

    pH optimum
    Salt optimum
    pH 9
    0.5 M NaCl
    Unit definition: One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25 ºC), 5 mM MgCl2, 500 mM NaCl.

    HS: 38229000
    Storage Temperature: -15 °C to -25 °C

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