Protein Sample Preparation
Efficient lysis of cells and tissues is a crucial step for the successful isolation and purification of specific proteins.
One of the most efficient buffers for the lysis of mammalian cells is RIPA buffer. Save time and labor by using SERVA's ready-to-use RIPA Buffer for extraction of cytoplasmic, membrane and nuclear proteins, suitable for many applications like Western Blotting, protein purification and protein assays.
For determination of protein activity, gel shift assays and other functional assays it is necessary to preserve the native structure of the proteins. SERVA's Mammalian Protein Extraction Kits provide a fast and easy method for the isolation of native total protein or native cytoplasmic, membrane and nuclear protein fractions from cells or tissues. The lysis buffers allow for mild but efficient lysis with high protein yield.
As a complement SERVA offers:
- Application optimized protease and phosphatase inhibitor mixes for isolation of intact proteins
- A broad range of detergents for solubilization of membrane proteins
- Application proofed reagents for preparation of lysis buffers according to your needs
- Enzymes like lysozyme for lysis of bacteria
For many downstream applications is is necessary to remove contaminations like nucleic acids, salts and other low molecular weight substances after protein isolation. , to exchange buffer or concentrate the sample after protein isolation.
SERVA has a solution for every task:
- Cyanase Nuclease™ - fastest nuclease on the market for reduction of sample viscosity caused by nucleic acids prior to electrophoresis or chromatography
- Salt Active Nuclease - the only nuclease active in high salt buffers enabeling as well the digestion of protein-bound DNA
- CentriPure Gelfitration Columns - rapid and efficient desalting, buffer exchange and removal of small molecules
- Proteus X-Spinner Ultrafiltration Concentrators - One-step concentration and separation from low molecular weight substances, unique design allows as well efficient purification of membrane proteins
- Proteus Detergent Anion Exchange Mini Spin Column Kit - remove excess detergents and concentrate your protein in only 10 minutes
- SERVA BluePrep Major Serum Protein Removal Kit - fast and simple procedure for the effective depletion of albumin, alpha-antitrypsin, transferrin and haptoglobulin from serum and plasma samples prior to 2D SDS-PAGE
- Proteus NoEndo™ Kits - for endotoxin decontamination of biotherapy products
Recombinant proteins, labelled proteins or antibodies are easily purified by affinitity chromatography with high purity and yield.
SERVA's standard and Superflow agarose resins in various formats (loose, pre-packed columns, kits) and magnetic agarose beads enable protein purification from lab to process scale.
I. His-Tag purification by Immobilized Metal Affinity Chromatography (IMAC)
- SERVA IMAC Agarose Resins (Ni-NTA/Ni-, Co-, Zn- & Cu-IDA) - for low pressure gravity chromatography in batch or column format
- Super Ni-, Co-NTA Agarose Resins - for high pressure column chromatography in FPLC, as well in process scale
- Ni-NTA Magnetic Agarose Beads - for fast screening in small volumes, automation compatible
- Ni-IMAC Mini Kits - fast and simple spin column chromatography for high throughput
II. GST-Tag purification
- Glutathione Agarose Resin - rapid, highly selective one-step purification in batch or column format
III. Antibody purification
- Recombinant Protein A and Protein G Sepharose Resins - for high pressure column chromatography in FPLC, as well in process scale
- Proteus Protein A and Protein G Mini Kits - fast and simple spin column cromatography for high throughput
IV. Purification of biotinylated biomolecules
- SERVA Streptavidin Agarose - highly selective one-step purificationprovides because of high binding capacity and lower non-specific binding
Of course, you will as well find on our web site all necessary reagents for the preparation of binding and elution buffers, like e.g. imidazole, solvents for precipitation steps or other required chemicals in the section reagents.