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HOME Information Center Enzymes in Mass Spectrometry Advantages of Enzymatic Protein Digest

Advantages of Enzymatic Protein Digest

  • Only catalytic amounts of proteases necessary
  • High specificity
         Trypsin (EC no. 3.4.21.4): Lys/Arg (NH4HCO3, pH 8.0)
         Glu-C (EC no. 3.4.21.19): Glu (NH4HCO3, pH 7.8)
         Lys-C (EC no. 3.4.21.50): Lys (50 mM Tris/HCl pH 8.5)
  • High cleavage yield

  • Negligible side reaction
  • High quality proteases are commercially available
Proteases for Protein Digestion
Advantages of Enzymatic Protein Digest
Protocols
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SERVA Catalog
2023
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Electrophoresis by SERVA - Catalog
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SERVA Catalog
2023

In detail you will find the following new products in catalog 2022:

• New glycosidases such as Endo F3, Endo S and Sialidase
• Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
• New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
• BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
• he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
• Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
SERVA Catalog 2023.pdf
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Electrophoresis by SERVA - Catalog

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
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Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

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