SDS PAGE

The mostly used electrophoresis method for protein separation and analysis is the SDS polyacrylamide gel electrophoresis (SDS PAGE). The sample and the running buffer contain the detergent sodium dodecylsulfate (SDS).

The proteins and the SDS form micelles which migration speed in the gel matrix is relative to the molecular weight of the proteins. Due to this, the SDS PAGE allows separation of proteins according to the molecular weight. 

PRIME-Pakete 2017

Furthermore, all proteins have a negative net charge and migrate towards the anode (+). The proteins are separated in a polyacrylamide gel matrix.

The separation performance and resolution of the gel can be varied by the acrylamide concentration and the running buffer.

After separation, the protein bands are detected by staining either with colorimetric (Coomassie®, silver) or fluorescent dyes.

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September - December 2019

• Protein Electrophoresis - Reagents and Equipment
• Western Blotting
• Mini Vertical 2D Electrophoresis
• DNA/RNA-Elektrophoresis
• Micro- and Molecular Biology
• Dextrans & PEG, PVP
• Antibiotics
• Apoptosis
• Reagentis for Microskopy

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Product of the Month September
Ni-Extrachel™ Agarose Resin

A chemically resistant resin for purification of recombinant polyHis-tagged proteins.

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