Collagenase NB Grades
Collagenase NB 4 Standard Grade (cat. no. 17454) contains collagenase class I and class II as well as a balanced ratio of proteolytic side activities like neutral protease or clostripain. It is suitable for mild and effective isolation of a broad variety of cells.
Collagenase NB 4G Proved Grade (cat. no. 17465) possesses an increased collagenase activity compared to Collagenase NB 4 Standard Grade and a balanced mix of neutral protease, clostripain and trypsin-like activities.
Collagenase NB 5 Sterile Grade (cat. no. 17459) has similar enzymatic properties as Collagenase NB 4 Standard Grade, but is manufactured under aseptic conditions according to the European Pharmacopoeia.
Collagenase NB 8 Broad Range (cat. no. 17456) is a purified collagenase characterized by an increased collagenase activity and reduced levels of proteolytic side activities and bacterial endotoxins.
Collagenase NB 1 Premium Grade (cat. no. 17455) is a chromatographically highly purified product. It contains collagenase class I and II and very low levels of proteolytic side activities and bacterial endotoxins. This enzyme is used for isolation of sensitive cells and guarantees high yields and viability. It is especially applicable to isolation of pancreatic islets of Langerhans from different species like human or pig.
Chromatographically purified Neutral Protease NB (cat. no. 30301) is an
essential additive for isolation of pancreatic islets and is provided
separately allowing individual dosage and high stability of the enzymes.
With Neutral Protease NB High Active Grade (cat. no. 30302) a higher purified alternative of the standard Neutral Protease NB is available.
Good Manufacturing Practice for Cell Isolation
If isolated cells are used for tissue engineering or transplantation into humans collagenase (and neutral protease) are regarded as critical raw materials. Therefore these enzymes have to be manufactured in compliance with the EU guidelines to Good Manufacturing practice (GMP).
In addition SERVA GMP enzymes fulfill the requirements of TSE guidelines according to the European Pharmacopoeia.
Collagenase NB 6 GMP Grade (cat. no. 17458) is suitable for isolation of cells from a broad range of tissues. Among others it is optimal for isolation of human stem cells, ADSCs, hepatocytes or chondrocytes. It is sterile according to Ph. Eur.
The enzymatic properties are comparable to Collagenase NB 4 Standard Grade.
Collagenase NB 1 GMP Grade (cat. no. 17452) and Neutral Protease NB
GMP Grade (cat. no. 30303) are especially designed for isolation of islets
of for isolation of sensitive cells such as from pancreas or dental pulp.
Their enzymatic properties are comparable to Collagenase NB 1 Premium Grade and Neutral Protease NB, respectively.
Collagenase AF-1 GMP Grade (cat. no. 17457) and Neutral Protease AF GMP Grade (cat. no. 30306) are chromatographically purified enzymes manufactured free of any animal derived materials. These animal origin free enzymes have similar enzymatic properties to Collagenase NB 1 GMP Grade and Neutral Protease NB GMP Grade, and represent the first choice for clinical isolation of especially sensitive cells like pancreatic islets, hepatocytes and dental pulp.