Blotting

Nucleic acids are usually separated on agarose gels. Because of the consistency of the gel matrix, blotting can be performed as a capillary blot using SSC or SSPE as blotting buffer. The ideal transfer membrane is a nylon membrane with positive surface like Nylon-Bind B.
Proteins are most often separated by acrylamide gel electrophoresis. As these gels are too restrictive for capillary blotting, Western Blots are done by electro transfer.
Depending on the type of blotting (tank or semi-dry blotting) and the specific characteristics of the proteins, different buffers may be used for Western Blotting. For small proteins or for tank blotting after SDS PAGE Towbin Buffer is recommended, for blotting proteins after SDS PAGE or IEF by semi-dry blotting, a discontinuous buffer system is recommended. A fast and easy to use alternative for optimal semi-dry blotting is SERVA’s Xpress Blotting Kit. The buffer system enables in combination with the newly developed alternative for blotting paper, the Blotting Fleece, an efficient simultaneous transfer of high and low molecular weight proteins in only 15 minutes.
Although nitrocellulose membranes are still used for routine applications, the optimum result is achieved with PVDF membranes like Fluorobind membrane.
Fibre-reinforced nitrocellulose membranes are a good alternative to standard nitrocellulose membranes, because they allow an easier handling and cutting, stripping and repeated hybridization as well as automated immobilizing.
Detection of membrane bound antigens or nucleic acid sequences labelled with Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) can be done with colorimetric substrates like TMB or BCIP/NBT or chemiluminescent substrates.



News

Product of the Month December:
Xpress Blotting Kits

Autumn Campaign 2017 -
Great Saving!

Hoefer Promotion -
valid until 31.12.2017

BioDrop Promotion -
valid until 31.12.2017

Western Blotting - New Brochure!

Top
Close window
Product of the Month December:
Xpress Blotting Kits

Only 15 Minutes for Semi-Dry Western Blotting

This way to the Special Offer

Close window
Autumn Campaign 2017 -
Great Saving!
  • PRiME™-Packages for Minigel SDS PAGE
  • Protein Electrophoresis
  • Nucleic Acid Electrophoresis
  • Western Blotting
  • Laboratory Equipment
  • Trypsin MS Approved
  • DNA Decon

This way to the campaign

Close window
Hoefer Promotion -
valid until 31.12.2017

Save 25 % :

  • Mini Vertical Electrophoresis Units
  • Standard Vertical Electrophoresis Units
  • Tank Blotter

This way to the Promotion

Close window
BioDrop Promotion -
valid until 31.12.2017

BioDrop UV/VIS Spectrophotometer at a Glance:

  • BioDrop TOUCH
  • BioDrop µLITE
  • BioDrop DU

This way to the promotion!

 

Close window
Western Blotting - New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting

Broschüre (PDF)

Close window
SERVA - now on YouTube

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.