Detergents are ampiphilic substances containing at least one hydrophilic (polar) and one hydrophobic (lipophilic, non-polar) functional group. According to the ionic character of the polar group, they are classified as ionic (positively or negatively charged), non-ionic (uncharged) or zwitterionic (positively and negatively charged) detergents. You will find further information about properties and applications of detergents in our section Information Center.
Detergents are components of lysis buffers, wash buffers for Western Blots and ELISA. They are used for solubilization, stabilization, reconstitution and crystallization of proteins. Substances used for these applications like e.g. SDS, sodium deoxycholate or Tween 20 are application-tested in our QC and hence guarantee best results.
To achieve optimal results in proteomic applications the quality of used
detergents is crucial. Moreover, depending on the sample, different
combinations of zwitterionic detergents must be applied for efficient
solubilization of proteins and protein complexes.
Thus, SERVA offers apart from well-known detergents like CHAPS or Triton X-100, detergents like ASB-14 or ASB-16, which show superior protein solubilization properties, especially in 2D gel electrophoresis.