Protein Extraction Kits

RIPA buffer is a very effective buffer for lysis of cultured mammalians cells. It enables protein extraction from cytoplasmic, membrane and nuclear proteins, suitable for many applications like Western Blotting, protein purification and protein assays.
Since the buffer will disrupt protein-protein interactions, applications like immunoprecipitation and pull-down assays may be disturbed.

If for determination of protein activity and other functional assays integrity of protein structure is needed, SERVA's Mammalian Protein Extraction Kits allow the fast and easy preparation of total protein or cytoplasmic, membrane and nuclear protein fractions from cells or tissues.

  • Mild but efficient lysis with high protein yield
  • Performance is optimized and validated for several cell lines and tissues
  • No need for mechanical disruption
  • Reagents are compatible with standard protein quantification assays like BCA Protein Assay
  • Extracted proteins are directly applicable in downstream applications like Western Blot, ELISA, EMSA



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    SERVA Catalog
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    In detail you will find the following new products in catalog 2019:

    • • SERVA TAE Clear G Agarose tablets for practical nucleic acid electrophoresis
    • • Novel enzymes such as recombinant PNGases F as lyophilized powder
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    • • New buffers for molecular and cell biology
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    • • Electrophoresis gel and blot automation: SERVA BlueStain is an innovative device for the treatment of automated gel and blot staining
    SERVA Catalog 2019.pdf
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    Western Blotting -
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    • • Molecular Weight Standards
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    SERVA Brochures: Western Blotting V1806 (pdf)
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