Sample Preparation

Mammalian Total Protein Extraction Kit - Fast and Simple Isolation of Native Cytoplasmic, Membrane and Nuclear Proteins

  • Contains ready-to-use, optimized extraction buffer and protease inhibitor cocktail 
  • For protein isolation from cells and tissues
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Mammalian Membrane Protein Extraction Kit - Fast and Simple Isolation of Native Membrane Proteins

  • Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail 
  • Simple and effcient isolation of membrane and cytoplasmic protein fractions from cells and tissues within 75 min
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Mammalian Nuclear and Cytoplasmic Protein Extraction Kit - Fast and Simple Isolation of Native Nuclear and Cytoplasmic Proteins

  • Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail 
  • Simple and effcient isolation of nuclear and cytoplasmic protein fractions from cells and tissues within 80 min
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Recombinant TEV Protease for Efficient Removal of Affinity Tags from Fusion Proteins

  • Highly site-specific cysteine protease
  • Genetically modified to increase activity and resistance to autolysis
  • Contains a N-terminal polyhistidine tag for easy removal from the cleavage reaction by affinity chromatography
  • Supplied with 1 ml 20x TEV Reaction Buffer and 100 mM DTT
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Lysyl Endopeptidase®, MS approved - For Improved Sequence Coverage in Mass Spectrometry Analysis

  • Cleaves proteins with high specificity and efficiency at the C terminus of lysine
  • High activity optimized for in-gel digestion
  • Increased number of peptides after double digest with trypsin due to improved cleavage at lysine residues
  • Higher sequence coverage allows for easy database research by peptide mass
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Recombinant PNGase F - The Choice for Use in MALDI-Imaging due to Faster Kinetics and Greater Activity against Native Proteins

  • Mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli
  • Specifically removes N-linked oligosaccharides from glycoproteins
  • Does not need a denaturing step
  • Works on native glycoproteins and serum glycoproteins in minutes at room temperature
  • Digestion leads to a more complete glycan release as compared to other commercially-available enzymes
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Cyanase™ Nuclease - Your Alternative for Benzonase®

  • Digestion of single and double stranded DNA and RNA in as little as 1 minute
  • Active in higher salt concentrations and over a wide pH range
  • Unaffected by lysozyme, detergents or urea, DTT
  • In only 20 min with Cyanase™ Inactivation Resin removed from the reaction 
  • Unsurpassed stability, can be stored up to 1 year at room temperature

Benzonase = trademark of Merck KGaA, Cyanase = trademark of RiboSolutions, Inc.

Cyanase Nuclease

Salt Active Nuclease - Only Endonuclease with an Optimum Activity in 0.5 M NaCl

  • Digests as well protein-bound DNA due to optimum activity in 0.5 M NaCl 
  • Active at low temperatures and high pH range
  • Easily inactivated by denaturing reagents
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Recombinant Proteinase K - Suitable for Application in Sensitive PCR Reactions

  • Endopeptidase with very high specific activity and broad spectrum of action
  • Digestion of proteins in nucleic acids preparations without compromising integrity of isolated DNA or RNA
  • Improves cloning efficiency of PCR products
  • Free of endonucleases, exonucleases and ribonucleases
  • DNA: ≤10 pg/mg enzyme

 

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BlueZol - Ready-to-Use Reagent for the Rapid Isolation of RNA, DNA and Proteins from a Single Sample

  • For the rapid isolation of total RNA from cells and tissues of human, animal, plant, yeast or bacterial origin
  • Due to highly effective RNase inhibitory properties the integrity of the RNA during lysis is fully protected
  • Suitable for the simultaneous isolation of RNA, DNA and protein from one sample
  • Purified RNA is ideal for any downstream applications such as RT-PCR, in vitro translation, Northern Blotting, RNase protection assays or dot blot hybridization
  • Purified DNA can be used for PCR and Southern Blotting and the proteins for Western Blotting
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CTAB DNA Extraction Buffer for DNA Isolation from Plants

Ready-to-use buffer with the non-ionic detergent cetyltrimethylammonium-bromide (CTAB) for removal of polysaccharides contamination during isolation of DNA from plants. By adjustment of salt concentration in lysates containing CTAB, polysaccharides and DNA can be differentially precipitated.

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Recombinant Ribonuclease Inhibitor from Human Placenta

  • Completely inhibits RNAse A, RNase B and RNase C activity
  • No polymerase or reverse transcriptase activity
  • Stable up to 55 °C
  • Suitable for applications like in vitro translation, cDNA synthesis, RNA in vitro analysis, RNA purification
  • Free of RNases, Nickases, Exo- and Endonucleases

SERVA Ni-NTA Magnetic Beads for Fast and Easy Purification of His-Tagged Proteins

  • Ideal for small sample volumes
  • High binding capacity: > 75 mg His-tagged protein/ml gel
  • No need for protocol change – easy changeover from other resins
  • Suitable for native and denaturing conditions

SERVA Ni-NTA Agarose Resin - Rapid, Highly Selective One-Step Purification of His-Tag Proteins

  • High-protein binding and minimal metal leaching
  • Excellent chemical and mechanical stability
  • No need for protocol change  – easy changeover from other resins
  • Suitable for batch and column purification
  • Purification under native and denaturing conditions
  • Empty columns for small and larger volumes

SERVA Ni-, Co, Zn- and Cu-IDA Agarose Resins in Two Different Charge Densities - The Optimum Solution for Every Application

  • With high charge density for high binding capacity and low charge density for selectivity/specificity
  • Excellent chemical and mechanical stability
  • Ready-to-use pre-packed columns for fast and simple purification
  • Cost-effective bulk resins suitable for batch and column purification
  • Empty columns for small and larger volumes available
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Glutathione Agarose Resin - Rapid, Highly Selective One-Step Purification of GST-Tag Proteins

  • Fast and easy - high purity achieved with one purification step
  • Suitable for small and large protein complexes
  • Excellent chemical and mechanical stability
  • Easy changeover from other resins - no need for protocol change
  • For batch and column purification
  • Empty columns for small and larger volumes available
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Xpress Micro Dialyzer - Rapid Dialysis of Sample Volumes of 10 - 300 µl

  • High yield - up to 98 % sample recovery
  • Easy sample loading and retrieval with standard laboratory micropipettes
  • Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
  • Flexible – scalable from 1 to 96 samples, dialysis in micro tubes/microtiter plates or in beakers
  • Automation compatible – plate format conforms to the SBS Microplate Standard
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Xpress Mini Dialyzer - Rapid Dialysis of Sample Volumes of 150 µl - 1 ml

  • High yield - up to 98 % sample recovery
  • Easy sample loading and retrieval with standard laboratory micropipettes
  • Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
  • Flexible – scalable from 1 to 48 samples, dialysis in micro tubes/microtiter plates or in beakers
  • Automation compatible – plate format conforms to the SBS Microplate Standard
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ReadyLyzer - Ready-to-use Dialysis System for Quick and Efficient Dialysis and Buffer Exchange

  • Easily opened and closed with a screw cap
  • No struggling with closures and leaking knots or risk of puncturing the membrane
  • Delivered with a flotation ring for improved buoyancy and vertical orientation
  • Available volume sizes: 10 - 250 µl, 50 – 800 µl, 0.1 – 3 ml, 10 ml, 20 ml
  • High recovery 98 %
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Promotions

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September - December 2018

Product of the Month November:
Mammalian Membrane Protein Extraction Kit

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Market Square
September - December 2018

Electrophoresis:

  • Nucleic Acid Electrophoresis
  • SDS-PAGE
  •  Horizontal Isoelectric Focusing (IEF)
  •  Western Blotting: Reagents & Equipment

Life Sciences:

  • Enzymes & Reagents for Mass Spectrometry
  • Detergents
  • Antibiotics
  • Protein Quantification
  • Protease- & Phosphatase-Inhibitors

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Product of the Month November:
Mammalian Membrane Protein Extraction Kit

Fast, Easy and Gentle Protein Isolation

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