N-Lauroylsarcosine, Na-salt (30 % solution)

Sarcosyl surfactants can be considered as modified fatty acids, where the hydrocarbon chain is interrupted by an amidomethyl (-CONCH3-) group. The structure is similar to soaps, but due to the sarcosyl group, they show a better solubility in water and no sensitivity to calcium ions. The lauric acid in our product is of plant origin.

In biotechnology, N-Lauroylsarcosinate is mainly used for the isolation and characterization of DNA, proteins and enzymes, and for tissue homogenization. Diluted solutions of N-lauroylsarcosinate show at acid pH bacteriostatic properties.


Synonyms: N-Methyl-N-(oxododecyl)-glycine, sodium salt; Sarkosyl NL-30, Oramix L30

CAS registry number: [137-16-6]

Molecular Formula: C15H28NO3Na

Relative molecular mass: 293.4

Classification: Anionic surfactant

CMC: 13.7 mM


Bibliography

Extraction and Characterization of DNA and DNA Fragments

Hicks, K.E. et al. (1995) A simple and sensitive DNA hybridization assay used for the routine diagnosis of human Parvovirus B19 infection. J. Clin. Microbiol. 33, 2473-5.

Weiler, J.  et al. (1997) Hybridisation based DNA screening on peptide nucleic acid (PNA) oligomer arrays. Nucl. Acids Res. 25, 2792-9.

Nakamura, Y. et al. (1999) Expression of soybean b-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants. Plant Cell Rep. 18, 527-32.

Li, H. et al. (2001) A rapid and high yielding DNA miniprep for cotton (Gossypium spp.). Plant Mol. Biol. Rep. 19, 1-5.

Sun, L.V. et al. (2001) Determination of Wolbachia genome size by pulsed-field gel electrophoresis. J. Bacteriol. 183, 2219-25.

Cosa, G. et al. (2002) DNA damage detection technique applying time-resolved fluorescence measurements. Anal. Chem. 74, 6163-9.

Isolation and Investigation of RNA

MacDonald, R.J. et al. (1987) Isolation of RNA using guanidinium salts. Meth. Enzymol. 152, 219-227.

Kimura, H. et al. (1999) Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure. Mol. Cell. Biol. 19, 5383-92.

Suzuki, F. et al. (2002) Distribution of alpha-1 adrenoceptor subtypes in RNA and protein in rabbit eyes. Brit. J. Pharmacol. 135, 600-8.

Pant, B.D. et al. (2009) Identification of nutrient-responsive Arabidopsis and rapeseed microRNAs by comprehensive real-time polymerase chain reaction profiling and small RNA sequencing. Plant Physiol. 150, 1541-55.

Isolation and Characterization of Enzymes and Proteins

Magnuson, T.S. et al. (2000) Characterization of a membrane-bound NADH-dependent Fe3+ reductase from the dissimilatory Fe3+-reducing bacterium Geobacter sulfurreducens. FEMS Microbiol. Lett. 185, 205-11.

Bader, J.A. et al. (2004) Immune response induced by N-lauroylsarcosine extracted outer- membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish. Fish Shellfish Immunol. 16, 415-28.

Kahlau, S. a. Bock, R. (2008) Plastid transcriptomics and translatomics of tomatoe fruit development and chloroplast-to-chromoplast differentiation: chromoplast gene expression largely serves the production of a single protein. Plant Cell 20, 856-74.

Park, D.-W. et al. (2011) Improved recovery of active GST-fusion proteins from insoluble aggregates: solubilization and purification conditions using PKM2 and HtrA2 as model proteins. BMBRep.2011.44.4.279

Further Applications

Verbavatz, J.M. et al. (1993) Tetrameric assembly of CHIP28 water channels in liposomes and cell membranes: a freeze-fracture study. J. Cell Biol. 123, 605-18.

Rund, S. et al. (1999) Structural analysis of the lipopolysaccharide from Chlamydia trachomatis serotype L2. J. Biol. Chem. 274, 16819-24.

Piret, J. et al. (2002) Comparative study of mechanisms of Herpes simplex virus inactivation by sodium lauryl sulfate and N-lauroylsarcosine.

Langeveld, J.P.M. et al. (2003) Enzymatic degradation of prion protein in brain stem from infected cattle and sheep. J. Infect. Dis. 188, 1782-9.
Tamgüney, G. et al. (2009) Measuring prions by bioluminescence imaging. PNAS 106, 15002-6.


Top
Fenster schließen
Produkt des Monats Januar:
Protein Standard für SDS PAGE

SERVA ungefärbter Protein Standard 6,5 - 97 kDa: sehr gut geeignet für die Bestimmung des Molekulargewichts

→ Hier geht es zur Aktion

SERVA InfoMail - Erhalten Sie die neuesten Informationen

Fenster schließen

Mit der Anmeldung zu »SERVA InfoMail« erhalten Sie Informationen über neue Produkte, Werbeaktionen, Stellenangebote bei SERVA und vieles mehr. Dieser Service ist selbstverständlich kostenlos.
Sie können sich jederzeit wieder abmelden.

Schnelleinkauf

Fenster schließen

Mit der Funktion Schnelleinkauf können Sie mit nur einem Klick ein Produkt in Ihren Warenkorb legen. Geben Sie einfach die Kat.-Nr. wie im Katalog angegeben im Format xxxxx.yy ein und klicken Sie auf Go!