Classification of Detergents

Surfactants can be classified according to their charge, as follows:

Nonionic detergents contain uncharged hydrophilic head groups that consist of either polyoxyethylene moieties as in BRIJ or TRITON, PEG-sorbitan units as in TWEEN, or glycosidic groups as in octyl-ß-D-glucoside, dodecyl-ß-D-maltoside or digitonin. They are well suited for breaking lipid-lipid and lipid-protein interactions but normally do not break protein-protein interactions. Compared to ionic detergents, they usually do not denature proteins and are therefore frequently used for the isolation of biologically active membrane proteins.

Ionic detergents contain a head group which is either positively or negatively charged. For example, the anionic detergent sodium dodecyl sulfate (SDS) carries a negatively charged sulfate group on a linear C12 hydrocarbon chain. SDS is considered as a very strong and biologically harsh surfactant. It is able to denature proteins by breaking intra- and intermolecular interactions and thus destroying their biological activity. Other anionic detergents like the bile acid salts Na-cholate and Na-deoxycholate have a rigid steroidal core structure. They do not carry a well defined polar head group opposite to a hydrophobic tail (such as SDS). The polar groups are distributed on different parts of the molecule, resulting in a polar and non-polar side, e.g. Na-deoxycholate carries a carboxylate group at the end of a short hydrocarbon chain and two hydroxyl groups on the steroid structure. The bile acids are less denaturing than the ionic alkyl detergents, possibly due to their rigid steroidal ring structure.

Cationic detergents have a positively charged head group, which is often a quaternary ammonium group, e.g. CTAB carries a trimethylammonium group on a C16 hydrocarbon chain. It is also a strong detergent and will often irreversibly denature proteins.

Zwitterionic detergents like CHAPS or SB12 combine the properties of ionic and nonionic detergents. They carry a positively and a negatively charged group, but like nonionic surfactants, do not have a net charge (for CHAPS and sulfobetaines in the pH range of 2 -12). Furthermore they lack conductivity and electrophoretic mobility and do not bind to ion exchange resins. However, they are able - like ionic detergents - to break protein-protein interactions.

Detergents - Overview

Cloud Point

 Nonionic Detergents     

 Brij 35
 ca. 1200
 0.67 - 0.73
 Lubrol 17A17
 Synperionic F68
 ca. 8300
 Synperonic F108
 ca. 14000
 Triton X-100
 ca- 625
 63-69 (1 % in H2O)
 Tween 20
 76 (3 % in 1 N NaCl solution)
 Tween 80
 65 (3 % in 1 N NaCl solution

Ionic Detergents

 7 - 16
 2 - 7
 2.4 - 4
 2 - 19.9
 Na-Dioctyl sulfosuccinate

Zwitterionic Detergents

 4.2 - 6.5
 9 - 10
 SB 3 - 10
 25 - 40
 SB 3 - 12
 3 6

Fenster schließen
Produkt des Monats Januar:
Protein Standard für SDS PAGE

SERVA ungefärbter Protein Standard 6,5 - 97 kDa: sehr gut geeignet für die Bestimmung des Molekulargewichts

→ Hier geht es zur Aktion

SERVA InfoMail - Erhalten Sie die neuesten Informationen

Fenster schließen

Mit der Anmeldung zu »SERVA InfoMail« erhalten Sie Informationen über neue Produkte, Werbeaktionen, Stellenangebote bei SERVA und vieles mehr. Dieser Service ist selbstverständlich kostenlos.
Sie können sich jederzeit wieder abmelden.


Fenster schließen

Mit der Funktion Schnelleinkauf können Sie mit nur einem Klick ein Produkt in Ihren Warenkorb legen. Geben Sie einfach die Kat.-Nr. wie im Katalog angegeben im Format xxxxx.yy ein und klicken Sie auf Go!