Home › Trypsin NB Sequencing Grade, modified from porcine pancreas
Trypsin NB Sequencing Grade, modified from porcine pancreas
EC 3.4.21.4 • Mr
ca. 24 000
• CAS [9002-07-7]
Modified by reductive methylation, no chymotryptic activity detectable. Specificity
verified by digestion of oxidized B-chain of insulin. Trypsin NB
Sequencing Grade, modified, is suitable for digestion of proteins for mass
spectrometry analysis. Reductive methylation of the lysine residues results in
a stable product that is extremely resistant to autolytic degradation. Trypsin
NB Sequencing Grade, modified, is purified by chromatography. No chymotryptic
activity is detectable. Each package contains also a Trypsin Peptide (TP)
Standard allowing internal calibration for enhancing mass accuracy. The TP
standard provides tryptic activity to generate masses m/z 842 and 2211.
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