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Semi-dry blotting is faster than tank blotting. It requires less material and generates less heat. This has to do with the large volume of electrically conductive buffer used in tank blotting. Attempts to achieve high electric field strengths for rapid blotting lead to very high current demands and consequently large heat loads. These are not usually a factor in semi-dry blotting as the closely spaced electrodes separated by less conductive saturated filter papers permit rapid electrophoresis without the very high current. Both, discontinuous and continuous buffer systems can be applied. Discontinuous buffer systems are particularly recommended because transfer is very homogeneous for a wide range of molecular weights, even larger proteins up to 200 kDa can be transferred with an efficiency of > 80%. The BlueFlash™ Semi-Dry blotters are equipped with long-lasting electrodes made from synthetic carbon. They are resistant to corrosion (pH 3-9, SDS) and built for homogeneous, fast and efficient transfer of nucleic acids and proteins. The spring-mounted anode can be adapted to thicker gels (up to 5 mm) and gel stacks. The acrylic housing is resistant to 10 % ethanol and easy to clean. Safety lid, including design to prevent short-circuit (no gel). Little bench space required. Flexible Format and Application: Choose the right format for your application. SERVA offers semi-dry blotter units with various blotting areas ranging from small-sized BF-S and BF-M for mini gels up to large format BF-XXL to fit 2D gels. The sizes available suit all gel formats. They are suitable for blotting of large format gels (e.g. 2D) or simultaneous transfer of a series of mini gels. Usually, stacks of 2 gels can be blotted.
BlueFlash™ is a registered trademark of SERVA.

Blotting area: Operating range of constant current: Power supply requirements: Buffer volume: Apparatus dimensions:

150 x 150 mm 0.8 - 3.5 mA 200 V, 500 mA 0.2 ml/cm² x no. of sheets + 1.0 ml/cm2 of membrane + 1.5 ml/cm² of gel 28 x 20 x 8.3 cm

HS: 90272000
Storage Temperature: +15 °C to +30 °C

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Friday, May 25, 2012

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