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Alkaline Phosphatase from calf intestine ca. 3000 U/mg protein (ca. 60 U/µl)
solution
(Orthophosphoric-monoester phosphohydrolase (alkaline optimum))
EC 3.1.3.1. • Mr ca. 140 000 •
EC 3.1.3.1. • Mr ca. 140 000 •
XN
Harmful
R: 42
S: 23D - 36 - 45
• WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C
Especially suitable for the preparation of EIA-conjugates. Further dialysis is unnecessary (1). In 50 % glycerol, containing 5 mM Tris, 5 mM MgCl2,
0.1 mM ZnCl2, pH ca. 7.0.
Unit definition: 1 U catalyzes the hydrolysis of 1 µmole of 4-nitrophenyl phosphate per minute at 37 °C, pH 9.8 (DEA buffer) (6).
Activity in other units: ca. 1100 U/mg at 25 °C, pH 9.6 (glycine buffer)
Substrates for Alkaline Phosphatase:
4-Nitrophenyl phosphate·Na2-salt (cat.no. 30770) Phenolphthalein diphosphate·Na4-salt (cat.no. 32050) 5-Bromo-4-chloro-3-indoxyl-phosphate-p-toluidine-salt (BCIP)
(cat.no. 15247) 5-Bromo-4-chloro-3-indoxyl-phosphate·Na2-salt (cat.no. 15259) Naphthol-AS-BI-phosphate (cat.no. 29988) Naphthol-AS-MX-phosphate (cat.no. 30002) 1-Naphthyl phosphate·Na-salt (cat.no. 30130)
References:
- Chaconas, G. & van de Sande, J.H. (1980) Methods Enzymol. 65, 75-85
- Maxam, A.H. & Gilbert, W. (1980) Methods Enzymol. 65, 499-560
- Williams, D.G. (1984) J. Immunol. Methods 72, 261-8
- Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
- Garen, A. & Levinthal, C. (1960) Biochim. Biophys. Acta 38, 470-83
- Mössner, E. et al. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 543-9
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 32471.01 | 1 mg | inquire* | |
| 32471.02 | 5 mg | inquire* | |
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