Especially suitable for the preparation of EIA-conjugates. Further dialysis is
unnecessary (1). In 50 % glycerol, containing 5 mM Tris, 5 mM MgCl2,
0.1 mM ZnCl2, pH ca. 7.0.
Unit definition: 1 U catalyzes the hydrolysis of 1 µmole of
4-nitrophenyl phosphate per minute at 37 °C, pH 9.8 (DEA buffer) (6). Activity in other units: ca. 1100 U/mg at 25 °C, pH 9.6 (glycine buffer) Substrates for Alkaline Phosphatase: 4-Nitrophenyl phosphate·Na2-salt
(cat.no. 30770)
Phenolphthalein diphosphate·Na4-salt (cat.no. 32050)
5-Bromo-4-chloro-3-indoxyl-phosphate-p-toluidine-salt (BCIP) (cat.no. 15247)
5-Bromo-4-chloro-3-indoxyl-phosphate·Na2-salt (cat.no. 15259)
Naphthol-AS-BI-phosphate (cat.no. 29988)
Naphthol-AS-MX-phosphate (cat.no. 30002)
1-Naphthyl phosphate·Na-salt (cat.no. 30130)
References:
Chaconas, G. & van de Sande, J.H. (1980) Methods Enzymol. 65, 75-85
Maxam, A.H. & Gilbert, W. (1980) Methods Enzymol. 65, 499-560
Williams, D.G. (1984) J. Immunol. Methods 72, 261-8
Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
Garen, A. & Levinthal, C. (1960) Biochim. Biophys. Acta 38, 470-83
Mössner, E. et al. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361,
543-9
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H334
Deutsch:
Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.
English:
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Français:
Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.
Italiano:
Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Español:
Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.
BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position
ruhigstellen, die das Atmen erleichtert.
English:
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
Français:
EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.
Italiano:
IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
Español:
EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.
