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HomeEnzymologyEnzymes › Alkaline Phosphatase from calf intestine ca. 3000 U/mg protein (ca. 60 U/µl)

Alkaline Phosphatase from calf intestine ca. 3000 U/mg protein (ca. 60 U/µl)

solution
(Orthophosphoric-monoester phosphohydrolase (alkaline optimum))
EC 3.1.3.1.Mr ca. 140 000

Especially suitable for the preparation of EIA-conjugates. Further dialysis is unnecessary (1). In 50 % glycerol, containing 5 mM Tris, 5 mM MgCl2,
0.1 mM ZnCl2, pH ca. 7.0.

Unit definition: 1 U catalyzes the hydrolysis of 1 µmole of 4-nitrophenyl phosphate per minute at 37 °C, pH 9.8 (DEA buffer) (6).
Activity in other units: ca. 1100 U/mg at 25 °C, pH 9.6 (glycine buffer)
Substrates for Alkaline Phosphatase:
4-Nitrophenyl phosphate·Na2-salt (cat.no. 30770) Phenolphthalein diphosphate·Na4-salt (cat.no. 32050) 5-Bromo-4-chloro-3-indoxyl-phosphate-p-toluidine-salt (BCIP)
(cat.no. 15247) 5-Bromo-4-chloro-3-indoxyl-phosphate·Na2-salt (cat.no. 15259) Naphthol-AS-BI-phosphate (cat.no. 29988) Naphthol-AS-MX-phosphate (cat.no. 30002) 1-Naphthyl phosphate·Na-salt (cat.no. 30130)


References:
  1. Chaconas, G. & van de Sande, J.H. (1980) Methods Enzymol. 65, 75-85
  2. Maxam, A.H. & Gilbert, W. (1980) Methods Enzymol. 65, 499-560
  3. Williams, D.G. (1984) J. Immunol. Methods 72, 261-8
  4. Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
  5. Garen, A. & Levinthal, C. (1960) Biochim. Biophys. Acta 38, 470-83
  6. Mössner, E. et al. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 543-9
 XN 
Harmful

R: 42
S: 23D - 36 - 45

WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C


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32471.01 1 mg inquire*  
32471.02 5 mg inquire*  
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Sicherheitsdatenblatt
Material Safety Data Sheet

Saturday, September 4, 2010

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R 42

Deutsch:

Sensibilisierung durch Einatmen möglich.

English:

May cause sensitisation by inhalation.

Français:

Peut entraîner une sensibilisation par inhalation.

Italiano:

Può provocare sensibilizzazione per inalazione.

Español:

Posibilidad de sensibilización por inhalación.

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S 36

Deutsch:

Bei der Arbeit geeignete Schutzkleidung tragen.

English:

Wear suitable protective clothing.

Français:

Porter un vètement de protection approprié.

Italiano:

Usare indumenti protettivi adatti.

Español:

Úsese indumentaria protectora adecuada.

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S 45

Deutsch:

Bei Unfall oder Unwohlsein sofort Arzt zuziehen (wenn möglich, dieses Etikett vorzeigen).

English:

In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Français:

En cas d'accident ou de malaise, consulter immédiatement un médecin (si possible, lui montrer l'étiquette).

Italiano:

In caso di incidente o di malessere consultare immediatamente il medico (se possibile, mostrargli l'etichetta).

Español:

En caso de accidente o malestar, acúdase inmediatamente al médico (si es posible, muéstresele la etiqueta).

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