SERVALight Helios CL HRP WB Substrate Kit


Extremely sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin.
The substrate is readily prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a one-to-one ratio.
Due to the extremely high light output of the SERVALight Helios substrate very short exposure times or very high dilution of antibodies can be used. Especially when using film detection this is critical to receive optimal performance.
0.1 ml substrate is sufficient for one cm2 membrane.
· Extreme sensitivity, low femtogram limit of detection
· Long light emission for 8 hours
· Working solution stable for at least three days - reproducible results, less waste
· Primary antibody dilution 1:5000 -1:100.000
· Secondary antibody dilution 1:100.000 – 1:500.000
· Detection can be done preferably by CCD imaging equipment or film




HS: 38229000
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
42587.01
20 ml
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42587.02
100 ml
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42587.03
200 ml
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SERVA Catalog
2023

Electrophoresis by SERVA - Catalog

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SERVA Catalog
2023

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
SERVA Catalog 2023.pdf
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Electrophoresis by SERVA - Catalog

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

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SERVA Electrophoresis Catalog (PDF)
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