Applications

Applications of Collagenase NB - Overview

SERVA offers high quality Collagenase NB products which are particularly suitable for cell isolation from various tissues. Stringent production standards result in reliable lot-to-lot consistency and excellent performance. SERVA Collagenase NB is the first choice for researchers aiming for high yield of viable cells.

In this section application details are presented for cell types which have been successfully isolated with SERVA Collagenase NB products:

Acinar cells Adipocytes Amniotic cells
Cardiomyocytes Chondrocytes Chorion cells
Endometrial cells Endothelial cells Epithelial cells
Fibroblasts Hepatocytes Islets of Langerhans
Lung cells Mastocytes Melanoma cells
Muscle cells Neurons Oocytes
Osteoblasts Schwann cells Stem cells
Tumor cells Miscellaneous  

 

If your cell type is not listed please contact us for more protocol details!
We aim to extend our protocol database to give you our best support. Send us your cell isolation data with Collagenase NB and get a reward!

General remarks on Isolation Conditions
In general,  the required Collagenase NB concentration depends on tissue type and origin as well as isolation procedure. Suggested concentrations should be considered as starting points and progress of the digestion process should be monitored visually in order to determine the optimal conditions.
We recommend dosing Collagenase NB by activity (PZ units), not by weight. SERVA guarantees a high lot-to-lot consistency; however, small variations may occur. Therefore dosing in activity ensures consistent results for your cell isolation process.
For further information on optimization of tissue digestion please visit our support section!

Isolation of cells intended for transplantation into humans
Enzymes for tissue digestion are critical raw materials for tissue engineering and transplantation into humans. SERVA offers superior GMP Grade Collagenase NB products which are manufactured according to current GMP guidelines by the German pharmaceutical company Nordmark.  For further information please contact us!


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BlueNews: Issue June 2016.pdf
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New Application Note:
ICPL™ - Coupled Immunoprecipitation
(ICPL-IP)

ImmunoPrecipitation (IP) is widely used as a method to selectively isolate protein complexes from primary tissues. However, due to lack of specificity and selectivity of most antibodies and unspecific binding of the carrier beads, the method can produce false positives. The ICPL approach, employing stable isotope labelling and mass spectrometry, can discriminate true positive from false positive IP complexes.

SERVA ICPL™ Quadruplex Plus Kit 39233
Application Note: ICPL-IP
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