Hyaluronidase from ovine testes min. 300 U/mglyophil.
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Glucosidase which cleaves endo-N-acetlyhexosaminic bonds in hyaluronic acid
and chondroitin sulfate A and C to tetrasaccharide residues. As hyaluronic
acid and chondroitin sulfate are often found in connective tissues,
Hyaluronidase is often used in conjunction with collagenase to dissociate the
extracellular matrix between cells of animal tissue, in order to release
viable cells for use in tissue culture.
It may also be used to clarify synovial fluids in order to make cell counts possible.
Unit definition: The amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37 °C and pH 4.0.
- Dorfman, A (1955) Methods Enzymol. Vol. I, 166-173
EINECS: 253-464-3 • HS: 35079090
Storage Temperature: -15 °C to -25 °C