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Mr 85.000 •
Sialidases are a family of exoglycosidases that catalyze the cleavage of non-reducing sialic acid residues of mono- or oligosaccharide chains on glycoconjugates. SERVA Sialidase, a recombinant glycosidase from Arthrobacter ureafaciens, cleaves α2,3-, α2,6- and α2,8- linked sialic acids. Because of its broad substrate specificity, Sialidase is capable of completely removing sialic acids from glycoconjugates of a wide variety of biological materials (cells, antibodies, serum, tissues etc.).
Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature. Concentration after reconstitution: 50 u/µl in 100 µl H2O dest.
Unit definition: Denatured alpha-1-antitrypsin (A1AT) (10μg) is incubated with 1 µL of reconstituted Sialidase for 60 minutes at 37 °C and then analyzed by SDS-PAGE and analysis with the sialic acid binding Sambucus nigra lectin (SNA).
Storage Temperature: +15 °C to +30 °C