Endoproteinase Glu-C, recombinantsequencing grade
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220.127.116.11 • Mr ca. 24 000 • CAS [66676-43-5]
Due to its highly specific cleavage of peptides the serine protease Glu-C (S. aureus V8) is used to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.
The specificity of Glu-C is primarily determined by the buffer pH and composition. Using phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds. Ammonium bicarbonate buffer (pH 7.8) will lead to a preferential cleavage of glutamyl bonds. The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage.
- High specificity, purity and stability
- Recombinant - animal origin material
free, consistent lot-to-lot quality
Storage Temperature: -15 °C to -25 °C