dsDNase, heat labilesolution

Mr 47 600

Unique double-strand specific endonuclease, which can be easily inactivated by heat treatment. As it does not digest ssDNA or RNA, it allows to specifically remove dsDNA in the presence of other nucleic acids. The yielding product is oligonucleotides with 5’-phosphates and 3’-hydroxyl termini.
Recombinantly produced in Pichia pastoris, specific activity ca. 200 000 units/mg.

  • Irreversible inactivation by heat treatment at 5 - 15 min at 55 ˚C, 1 mM DTT, pH ≥ 8
  • Decontamination of PCR master mixes
  • Removal of genomic DNA from RNA preparations prior to RT-qPCR



    One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2


    HS: 35079090
    Storage Temperature: -15 °C to -25 °C

  • Cat.No.
    Size
    EUR
    18545.01
    250 U
    inquire*
    Please Login to order.
    *Please use the inquiry form in the right column.
    Dry Ice Shipment

    Certificates of Analysis (Lot.-no. - release date)

    PDF Documents

    You may find this also interesting:

    Product Inquiry
    *
    *
    *
    *Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
    Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

    Promotions

    SERVA Market Square 2021: April - June

    Product of the Month June
    Cellulase & Macerozyme

    Top
    Close window
    SERVA Market Square 2021: April - June

    Electrophoresis:

    • Vertical Protein Electrophoresis
    • Horizontal Protein Electrophoresis
    • Western Blotting
    • Electrophoresis Reagents
    • DNA/RNA Electrophoresis

    Life Sciences:

    • Fluorescence Labelling
    • Albumins
    • Protease and Phosphagtase Inhibitors
    • Enzymes & Reagents for Mass Spectrometry
    • Enzymes for Life Sciences

     

    → This way to the Special Offer

    Close window
    Product of the Month June
    Cellulase & Macerozyme

    For preparation of plant protoplasts

    → This way to the Special Offer

    SERVA InfoMail - Get the latest information

    Close window

    When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
    You may remove your name any time from the list of recipients.

    Quick Order

    Close window

    Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.