dsDNase, heat labilesolution
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Unique double-strand specific endonuclease, which can be easily inactivated by
heat treatment. As it does not digest ssDNA or RNA, it allows to specifically
remove dsDNA in the presence of other nucleic acids. The yielding product is
oligonucleotides with 5’-phosphates and 3’-hydroxyl termini.
Recombinantly produced in Pichia pastoris, specific activity ca. 200 000 units/mg.
One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2
Storage Temperature: -15 °C to -25 °C