Running buffer preparation

Running Buffer Preparation

Laemmli Buffer for SDS PAGE

Components of  10x Tris-Glycine/SDS (Laemmli) Buffer, pH 8.6

Components Cat. No. Concentration Amount
Tris 37181 0.25 M 30 g/L
Glycine 23391 1.92 M 144 g/L
SDS  20771 1 % 10 g/L


• Tris-Tricine/SDS Running Buffer

Components of  20x Tris-Tricine/SDS Running Buffer, pH 8.5

Components Cat. No. Concentration Amount
Tris 37181 1.2 M 145 g/L
Tricine 37196 0.8 M 143 g/L
SDS  20771 2 %  20 g/L


Important: Never adjust the pH value of the running buffers.

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