Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA.

Unit definition: 1 unit is that amount of activity which is capable of causing within 1 minute a decrease in absorbance at 300 nm equivalent to the maximum possible change in a 0.05 % solution of yeast RNA at 25 °C, pH 5.0.


References:

1. Kunitz, M. (1946) J. Biol. Chem. 164, 563-8
2. Krupp, G. & Gross, H.J. (1979) Nucl. Acids Res. 6, 3481-90
3. Levy, C.C. & Kaepetzky, T.P. (1980) J. Biol. Chem. 255, 2153-9
4. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.81)
5. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (3.13.1, 5.5.2, 4.7.3, 7.3.8)

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EINECS: 232-646-6 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C