(POD, HRP, Donor: hydrogen-peroxide oxidoreductase) EC 220.127.116.11 • Mr ca. 40 000 • CAS [9003-99-0]
For the determination of peroxide (1). Used as an indicator enzyme in
reactions where peroxide is produced (2). For labelling antibodies in
ELISA (3, 4). RZ (= A 403/A 275) = 3.0.
Unit definition: 1 U catalyzes the decomposition of 1 µmole hydrogen
peroxide per minute at 25 °C, pH 7.0; reaction coupled with phenol-
aminoantipyrine (5). Activity in other units: min. 250 purpurogallin units/mg (1
purpurogallin unit catalyzes the oxidation of 1 mg pyrogallol to purpurogallin
in 20 seconds at 20 °C and pH 6.0. The purpurogallin is extracted and
determined spectrophotometrically at 420 nm (6). 1 mg purpurogallin requires
13.5 µmole peroxide, 1 purpurogallin unit corresponds to the decomposition of
12 µmoles peroxide at 25 °C.)
Meiattini, F. (1985) Methods of Enzymatic Analysis, (Bergmeyer, H.U., ed.) 3rd
Ed. vol. 7, p. 566-71
Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed. vol. 2,
Johnson Jr., R.B. (1980) J. Immunoassay 1, 27-37
Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
Gallati, H. (1977) J. Clin. Chem. Clin. Biochem. 15, 699-703
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