Endo F3, recombinantlyophilized

(Endo-β-N-acetylglucosaminidase F3, Endoglycosidase)
Mr 36.000

Endo F3, recombinant endo-β-N-acetylglucosaminidase F3 from Flavobacterium meningosepticum, cleaves in β(1-4) link in between the two core GlcNAcs of asparagine linked glycans. Endo F3 cleaves this link on core-fucosylated structures. The enzyme can be applied to workflows alone or in conjunction with PNGase F to allow for structural characterization of core-fucosylated glycans in tissues while maintaining spatial localization.

  • Especially designed and tested for mass spectrometry imaging and HPLC/UPLC
  • Contains a His-tag for easy removal by affinity chromatography

    Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
    Concentration after reconstitution with 100 µl H2O dest.: 8 u/µl in 1x PBS

    Unit definition: Human IgG (10 µg) is incubated with 1 µL of Endo F3 for 60 minutes at 37 °C and then analyzed by SDS-PAGE. Fully glycosylated IgG heavy chain migrates at approximately 50kDa. Loss of glycan results in a 2.5 kDa shift that can be observed following staining with Coomassie Brilliant Blue™.

    HS: 35079090
    Storage Temperature: +15 °C to +30 °C

  • Cat.No.
    100 µg
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