Endo S, recombinantlyophilized

(Endo-β-N-acetylglucosaminidase S, Endoglycosidase)
Mr 108.000

Endo S, a recombinant endo-β-N-acetylglucosaminidase S from Streptococcus pyogenes, plays a central role in glycoengineering strategies for the development of IgG antibodies with improved therapeutic efficacy. This enzyme will leave any human IgG with a single N-acetylglucosamine, with or without an attached fucose molecule.

  • Especially designed and tested for mass spectrometry imaging and HPLC/UPLC
  • Outstanding performance — produces in HPLC clean peaks with very high yields
  • Contains a His-tag for easy removal by affinity chromatography

    The endoglycosidase Endo S has a unique accuracy for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules. The enzyme hydrolyzes the β(1-4) linkage between the two core GlcNAcs of asparagine linked biantennary complex-type glycans of human IgG Fc regions.
    Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
    Concentration after reconstitution with 10 µl/50 µl/100 µl H2O dest.: 200 u/µl (1.0 mg/ml) in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. Delivered with 10x reaction buffer.

    Unit definition: One unit of reconstituted Endo S will catalyze the deglycosylation of 10 µg of IgG in 60 minutes at 37 °C. One unit is equal to 1 IUB milliunit.

    HS: 35079090
    Storage Temperature: +15 °C to +30 °C

  • Cat.No.
    10 µg
    Please Login to order.
    50 µg
    100 µg
    *Please use the inquiry form in the right column.
    Product Inquiry
    *Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
    Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.



    SERVA InfoMail - Get the latest information

    Close window

    When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
    You may remove your name any time from the list of recipients.

    Quick Order

    Close window

    Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.