(Muramidase, Mucopeptide-glycohydrolase, Mucopeptide N-acetyl-muramoylhydrolase) EC 188.8.131.52 • Mr
ca. 14 400
• CAS [12650-88-3]
Crystalline powder in hydrochloride form. Lysozyme hydrolyzes the β 1 → 4
linkages of the murein between N-acetylmuramic acid and N-acetyl-D-glucosamin,
and degrades the heteroglycan chain to disaccharides. This reaction leads to
cell lysis in most gram positive bacteria e.g. Micrococcus luteus. Lysis can
be prevented if the reaction is performed in an isotonic sucrose medium. Under
these assay conditions protoplasts are produced which no longer have a cell
wall. The lysis of Micrococcus luteus cells is used for the activity testing
of lysozyme. In gram negative bacteria a compact lipopolysaccharide layer on
the exposed murein sacculus efficiently shields them from lysozyme digestion.
Only when the stabilizing Ca2+ ions are removed by treatment with
e.g. EDTA, the murein becomes susceptible to lysozyme. Suitable for hydrolysis
of bacterial cell walls and of proteoglycans (1, 2, 4).
Unit definition: 1 unit catalyzes a decrease in absorption at 450 nm of
0.001 per minute at 25 °C, pH 7.0, in a suspension of Micrococcus luteus cells
(reaction volume 3 ml, light path 1 cm) (3).
Isoelectric point: 10.5 - 11.0
Optimum pH: 9.2
Imoto, T. et al. (1972) The Enzymes VII, 3rd Ed. (Boyer, P.D., ed.) Acad.
Press N.Y. 666-70
How to find the required certificate of analysis for your product: In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02. Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow. Now select the lot number from the list and download the PDF with a click. Done.
When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge. You may remove your name any time from the list of recipients.
Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.