SERVA PRiME Lightning Red


SERVA Lightning Red is a fluorescent dye for rapid labelling of proteins prior to SDS PAGE and/or Western Blotting, making any staining and washing steps after electrophoresis unnecessary. Normalization in Western Blots by using the total protein signal on the membrane as loading control can be easily done with the pre-labelled samples. In addition, the dye is fully compatible with mass spectrometry and other downstream methods.
The labelling procedure is simple and quick: 2 - 5 min for qualitative and 30 min for quantitative analysis.
For separation of the labelled proteins a wide range of gel formats including precast gels in plastic cassettes like SERVAGel PRiME Vertical Mini Gels and film-backed gels can be used.
The labelling works with complex and purified samples in a protein concentration range between 1 µg/ml up to 20 mg/ml - no need for protein concentration measurement or purification and concentration steps before or after labelling.

After the run, detection of labelled proteins is performed by fluorescent imager (camera or scanner) at an excitation wavelength of about 530 nm and emission filter of 610 nm with a narrow band width of 30 nm.
Depending on the used protocol sufficient for 250 - 1250 lanes - no matter what size of gel (mini, wide or large format) you use.

  • Simple and fast pre-labelling of proteins for SDS PAGE and Western Blotting
  • No need for protein concentration measurement, purification or concentration steps before or after labelling
  • No staining and washing steps after the run
  • Broad protein concentration range
  • Very high sensitivity, very low background
  • Wide dynamic and linear range
  • Compatible with all common gel electrophoresis buffer systems and mass spectrometry



HS: 38220000
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
43402.01
1 kit
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SERVA Catalog
2021

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Certificates of Analysis
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Western Blotting -
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New !

SERVA Catalog
2021

In detail you will find the following new products in catalog 2021:

  • • New enzymes like dsDNase for highly specific degradation of doubled-stranded DNA
  • • Zymolyase® to produce yeast protoplast or spheroplast
  • • Tergitol™ as an alternative to Triton X-100
  • • Easy Peel 2D HPE™ gels for blotting of horizontal 2D gels
  • • New protein standards, e.g. SERVA Unstained Protein Standard 6.5 - 97 kDa
  • • BlueVertical™ PRiME™ Blot Modul for SERVA’s BV-104 electrophoresis tank
  • • IPG tray with electrode lid for 1st dimension protein separation on 7 cm to 24 cm IPG strips
  • • Special Pharma Edition of SERVA BlueStain automated gel staining device

 

SERVA Catalog 2021 (pdf)
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Certificates of Analysis
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How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

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Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting V2104 (pdf)
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