PNGase F, recombinantsolution

Mr ca. 36 000

Recombinant glycosidase from Flavobacterium meningosepticum with faster kinetics and greater activity against native glycoproteins.

The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources:

  • Does not need a denaturing step
  • Works on native glycoproteins and serum glycoproteins in only minutes at room temperature
  • Leads to a more complete glycan release compared to other commercially-available enzymes
  • Especially designed and tested for mass spectrometry imaging of tissue samples
  • Contains a His-tag for easy removal by affinity chromatography

    The enzyme catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
    Concentration: 1000 u/µl (2.0 mg/ml), supplied in 1x PBS




    Unit definition: Achieves complete deglycosylation of 10 µg of RNase B incubated in 1x PBS with 1 µl of PNGase F for 5 - 10 min at 37 °C or room temperature.


    HS: 35079090
    Storage Temperature: -15 °C to -25 °C

  • Product Inquiry
    *
    *
    *
    *Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
    Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

    News

    SERVA Catalog
    2023

    Electrophoresis by SERVA - Catalog

    Watch the SERVA Video!

    Certificates of Analysis
    now online!

    Top
    Close window
    SERVA Catalog
    2023

    In detail you will find the following new products in catalog 2022:

    New glycosidases such as Endo F3, Endo S and Sialidase
    Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
    New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
    BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
    he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
    Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
    SERVA Catalog 2023.pdf
    Close window
    Electrophoresis by SERVA - Catalog

    • Isoelectric Focusing

    • SDS PAGE

    • 2D Electrophoresis

    • Native Electrophoresis

    • Western Blotting

    • Nucleic Acid Electrophoresis

    • Equipment

    SERVA Electrophoresis Catalog (PDF)
    Close window
    Watch the SERVA Video!

    We are SERVA! Watch to see where we are located and how we are serving scientists worldwide.

    SERVA Video

    Close window
    Certificates of Analysis
    now online!

    How to find the required certificate of analysis for your product:
    In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
    Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
    Now select the lot number from the list and download the PDF with a click. Done.

    Close window
    SERVA - now on YouTube

    SERVA InfoMail - Get the latest information

    Close window

    When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
    You may remove your name any time from the list of recipients.

    Quick Order

    Close window

    Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.