SERVA Tris-Glycine/LDS Sample Buffer (4x)


Modified, non-reducing sample buffer for SDS PAGE, delivered as 4x concentrate.

Lithium dodecyl sulfate (LDS) replaces the commonly used sodium dodecyl sulfate (SDS) in the triethanolamine buffered sample buffer. LDS prevents degradation of proteins during sample preparation and heating and stabilizes the pH of the sample during gel run. It does not crystallize at lower temperatures due to significantly better solubility than SDS. Hence, protein stability as well as band resolution is significantly enhanced by cooled gel runs.
For reducing conditions the sample buffer may be supplemented with 10 mM DTT or 5 % β-mercaptoethanol (end concentration 1x sample buffer).
Contains:
Triethanolamine buffered, 40 % glycerol, 4 % Ficoll® 400, 4 % LDS, 0.025 % phenol red and 0.025 % bromophenol.



DANGER
Hazard Statements H318
Precautions P280
Reaction P305 +P351 +P338 - P310

WGK: 1HS: 38220000
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
42525.01
10 ml
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New !

SERVA Catalog
2021

In detail you will find the following new products in catalog 2021:

  • • New enzymes like dsDNase for highly specific degradation of doubled-stranded DNA
  • • Zymolyase® to produce yeast protoplast or spheroplast
  • • Tergitol™ as an alternative to Triton X-100
  • • Easy Peel 2D HPE™ gels for blotting of horizontal 2D gels
  • • New protein standards, e.g. SERVA Unstained Protein Standard 6.5 - 97 kDa
  • • BlueVertical™ PRiME™ Blot Modul for SERVA’s BV-104 electrophoresis tank
  • • IPG tray with electrode lid for 1st dimension protein separation on 7 cm to 24 cm IPG strips
  • • Special Pharma Edition of SERVA BlueStain automated gel staining device

 

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Western Blotting -
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SERVA Brochures: Western Blotting V2104 (pdf)
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H318

Deutsch:

Verursacht schwere Augenschäden.

English:

Causes serious eye damage.

Français:

Provoque des lésions oculaires graves.

Italiano:

Provoca gravi lesioni oculari.

Español:

Provoca lesiones oculares graves.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

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P310

Deutsch:

Sofort GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

Immediately call a POISON CENTER or doctor/physician.

Français:

Appeler immédiatement un CENTRE ANTIPOISON ou un médecin.

Italiano:

Contattare immediatamente un CENTRO ANTIVELENI o un medico.

Español:

Llamar inmediatamente a un CENTRO DE INFORMACION TOXICOLOGICA o a un médico.