Analog of galactose, not split by β-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal
(5-bromo-4-chloro-indolyl-β-D-galactoside, cat. no. 15243) for detection of lac⁺ colonies. Dissolve in H₂0 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.

Purity (HPLC) [α] 20 °C/D (c=1 % in water) 1,4-Dioxane

min. 98.0 % -28.5 ° to -34.5 ° not detected

References:

1. Donner, J. et al. (1982) J. Biol. Chem. 257, 14826-9
2. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (4.33, 4.37-4.38, 1.8-1.9, 17.12-17.13, B.11)
3. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (1.4.3, 16.2.3, 1.15.1)

WARNING

Hazard Statements	H319
Reaction	P305 +P351 +P338

EINECS: 206-703-0 • WGK: 1 • HS: 29389090
Storage Temperature: -15 °C to -25 °C