Isopropyl-β-D-thiogalactopyranosideresearch grade, dioxane-free

(IPTG, Isopropyl-1-thio-β-D-galactopyranoside)
C9H18O5SMr 238.3CAS [367-93-1]

Analog of galactose, not split by β-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal
(5-bromo-4-chloro-indolyl-β-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.

Purity (HPLC)
[α] 20 °C/D (c=1 % in water)
1,4-Dioxane
min. 98.0 %
-28.5 ° to -34.5 °
not detected


References:
  1. Donner, J. et al. (1982) J. Biol. Chem. 257, 14826-9
  2. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (4.33, 4.37-4.38, 1.8-1.9, 17.12-17.13, B.11)
  3. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (1.4.3, 16.2.3, 1.15.1)
WARNING
Hazard Statements H319
Reaction P305 +P351 +P338

EINECS: 206-703-0WGK: 1HS: 29389090
Storage Temperature: -15 °C to -25 °C

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H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.