SERVA HPE™ Lightning Red

(Fluorescence labelling)

Highly sensitive fluorescent dye for rapid labelling of proteins prior to 2D PAGE, making any staining and washing steps after electrophoresis unnecessary. In addition the dye is fully compatible with mass spectrometry and other downstream methods like Western Blotting.
The labelling procedure is simple and quick:
Typically use 80 pmol SERVA HPE™ Lightning Red for labelling of 1 µg protein. Incubate at 0 °C for 15 min. The labelled protein solution is directly applied to an IPG strip via cup- or rehydration loading.
After the 2D run, detection of labelled proteins is performed by fluorescent imager (camera or scanner) at an excitation wavelength of about 530 nm and emission filter of 610 nm with a narrow band width of 30 nm.
SERVA HPE™ Lightning Red is compatible with all additives typically used for sample solubilization and protein extraction, including carrier ampholytes and reductants.
Alkalescent conditions are sufficient, it is not required to titrate the pH to a defined value.

  • Direct detection
  • No staining and washing steps after the run
  • Very high sensitivity, < 100 pg protein / spot
  • Wide dynamic and linear range of >104
  • No over-staining effects
  • Fully MS and Western Blotting compatible

    The kit contains 250 µg SERVA HPE™ Lightning Red dye and DMSO.





    HS: 38220000
    Storage Temperature: +2 °C to +8 °C

Product Inquiry
*
*
*
*Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

Promotions

SERVA Market Square: March - June 2020

Product of the Month June:
DNADecon, GermDecon & Detergent 7X®

Top
Close window
SERVA Market Square: March - June 2020

• Vertical Protein Electrophoresis
• Horizontal Protein Electrophoresis
• Western Blotting
• Fluorescent  Dyes
• DNA/RNA Electrophoresis
• Fluorescence Labelling
• Cell based Assays
• Enzymes
• Detergents
• Protease- and Phosphatase Inhibitors

  → This way to the Special Offer

Close window
Product of the Month June:
DNADecon, GermDecon & Detergent 7X®

Effective cleaning of laboratory equipment and surfaces in cell and tissue culture and molecular biology

→ This way to the Special Offer

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.