4',6-Diamidino-2-phenylindole·2HClanalytical grade
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(DAPI, 4′,6-Diamidino-2-phenylindole dihydrochloride)
C16H15N5·2HCl • Mr 350.25 • CAS [28718-90-3]
C16H15N5·2HCl • Mr 350.25 • CAS [28718-90-3]
4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) is a cell permeable fluorescent dye binding selectively to DNA. It binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable fluorescent complex.
DAPI has been used for demonstration of mycoplasmas and viruses in cells (1), for fluorescent chromosome staining (2) and as a dye for brain stem (3).
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Purity
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min. 98.0 %
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References:
- Russel, W.C. et al. (1975) Nature 253, 461-2
- Schweizer, D. (1976) Chromosoma (B.) 58, 307-24
- van der Kooy, D. & Kuypers, H.G.J.M. (1979) Science 204, 873-5
EINECS: 249-186-7 • WGK: 1 • HS: 29339980
Storage Temperature: +2 °C to +8 °C
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