4',6-Diamidino-2-phenylindole·2HClanalytical grade

(DAPI, 4′,6-Diamidino-2-phenylindole dihydrochloride)
C16H15N5·2HClMr 350.25CAS [28718-90-3]

4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) is a cell permeable fluorescent dye binding selectively to DNA. It binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable fluorescent complex.
DAPI has been used for demonstration of mycoplasmas and viruses in cells (1), for fluorescent chromosome staining (2) and as a dye for brain stem (3).

min. 98.0 %

  1. Russel, W.C. et al. (1975) Nature 253, 461-2
  2. Schweizer, D. (1976) Chromosoma (B.) 58, 307-24
  3. van der Kooy, D. & Kuypers, H.G.J.M. (1979) Science 204, 873-5

EINECS: 249-186-7WGK: 1HS: 29339980
Storage Temperature: +2 °C to +8 °C

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