(Katalase) EC 220.127.116.11 • Mr ca. 240 000 • CAS [9001-05-2]
Catalase is used for the removal of peroxides, the generation of oxygen and,
in coupled systems, for the determination of metabolites e.g. uric acid (1). A
very stable preparation particularly suitable for immobilization.
Unit definition: 1 U catalyzes the cleavage of 1 µmole hydrogen
peroxide per minute to water and oxygen at 25 °C, pH 7. The decrease in
hydrogen peroxide concentration can be followed spectrophotometrically at 240
Bartl, K. & Ziegenhorn, J. (1985) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 7, p. 134-46
Aebi, H.E. (1983) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 3, p. 277-86
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