2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-
1,3-propanediolanalytical grade
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C8H19NO5 • Mr 209.24 • CAS [6976-37-0]

Zwitterionic buffering substance for a pH range of 5.8 – 7.2 (1).
Bis-Tris is used in biochemistry and molecular biology and is a common component of many buffer systems for electrophoresis. Since it may form a complex with some common metals, such as Cu(II) and Pb(II), formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris is a substitute for the highly toxic buffer cacodylate.
Assay (titr.) A 1 cm/10 % in water 280 nm Iron (Fe) Lead (Pb) pH 10 % in water pKa 20 |
min. 99.0 % max. 0.1 max. 5 ppm max. 3 ppm 9.4 - 10.4 6.46 |
References:
- Daabo, M. & Bates, R. (1970) J. Phys. Chem. 74, 702-5
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WARNING |
Hazard Statements | H319 |
Precautions | P264 - P280 |
Reaction | P305 +P351 +P338 - P337 +P313 |
EINECS: 230-237-7 • WGK: 1 • HS: 29221900
Storage Temperature: +15 °C to +30 °C