Endo S, recombinantlyophilized
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Mr 108.000 •
Endo S, a recombinant endo-β-N-acetylglucosaminidase S from Streptococcus pyogenes, plays a central role in glycoengineering strategies for the development of IgG antibodies with improved therapeutic efficacy. This enzyme will leave any human IgG with a single N-acetylglucosamine, with or without an attached fucose molecule.
The endoglycosidase Endo S has a unique accuracy for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules. The enzyme hydrolyzes the β(1-4) linkage between the two core GlcNAcs of asparagine linked biantennary complex-type glycans of human IgG Fc regions.
Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
Concentration after reconstitution with 10 µl/50 µl/100 µl H2O dest.: 200 u/µl (1.0 mg/ml) in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. Delivered with 10x reaction buffer.
Unit definition: One unit of reconstituted Endo S will catalyze the deglycosylation of 10 µg of IgG in 60 minutes at 37 °C. One unit is equal to 1 IUB milliunit.
Storage Temperature: +15 °C to +30 °C