Methylene Blue

(Basic Blue 9, Methylene blue chloride, Methylthionine chloride)
C.I.52015C16H18N3S+·Cl-Mr 319.86CAS [61-73-4]

Standardized according to DIN 58981. Methylene blue is very sensitive to oxidation and usually contains demethylated products, called Azure A, B and C and thionine. For staining and detecting RNA in PAGE (as 0.1 % solution in water). Water soluble nuclear staining dye, mostly used for staining of blood cells.

λ max. (0.0001 % in water)
A 1 cm/λ max./0.0001 % in water

655 - 670 nm
min. 0.17



References:
  1. Herrini & Schmidt (1988) Rapid, reversible staining of Northern Blots prior to hybridization. BioTechniques 6, 196
WARNING
Hazard Statements H302
Precautions P264 - P270
Reaction P301 +P312 - P330

EINECS: 200-515-2WGK: 2LHS: 32049000
Storage Temperature: +15 °C to +30 °C

Cat.No.
Size
EUR
29198.01
25 g
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H302

Deutsch:

Gesundheitsschädlich bei Verschlucken.

English:

Harmful if swallowed.

Français:

Nocif en cas d'ingestion.

Italiano:

Nocivo se ingerito.

Español:

Nocivo en caso de ingestión.

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P264

Deutsch:

Nach Gebrauch … gründlich waschen.

English:

Wash … thoroughly after handling.

Français:

Se laver … soigneusement après manipulation.

Italiano:

Lavare accuratamente … dopo l’uso.

Español:

Lavarse … concienzudamente tras la manipulación.

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P270

Deutsch:

Bei Gebrauch nicht essen, trinken oder rauchen.

English:

Do no eat, drink or smoke when using this product.

Français:

Ne pas manger, boire ou fumer en manipulant ce produit.

Italiano:

Non mangiare, né bere, né fumare durante l’uso.

Español:

No comer, beber ni fumar durante su utilización.

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P301 +P312

Deutsch:

BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.

Français:

EN CAS D’INGESTION: appeler un CENTRE ANTIPOISON ou un médecin en cas de malaise.

Italiano:

IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI o un medico.

Español:

EN CASO DE INGESTIÓN: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico si se encuentra mal.

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P330

Deutsch:

Mund ausspülen.

English:

Rinse mouth.

Français:

Rincer la bouche.

Italiano:

Sciacquare la bocca.

Español:

Enjuagarse la boca.