Cholesterol is a key component of eukaryotic cell membranes and plays a critical role in membrane organization, fluidity, and function. In cholesterol-rich lipid raft, numerous membrane proteins and important membrane activities, including those involved in signal transduction, are found.
In additiopn to the effect of cholesterol on membrane structure and function, the interaction of membrane proteins with cholesterol have been reported. Many membrane proteins, such as G-protein coupled receptors (GPCRs), cholesterol binding proteins, and amyloid precursor protein require cholesterol binding to have their proper biological function.
Therefore cholesterol-based surfactants have become highly sought after. Cholesteryl hemisuccinate is a water-soluble cholesterol derivative that mimics native cholesterol function in cell membrane systems.
Cholesterol hemisuccinate and Lauryl Maltose Neopentyl Glycol (cat. no. 60110) have been shown to easily exchange membrane proteins into Lipid-Cubic Phase (LPC). Commonly used in combination with N-Dodecyl-β-D-Maltopyranoside (DDM, cat. no. 60099) for extracting membrane proteins.
Solubility: 1.2 % in a 6 % water solution of CHAPS

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