PNGase F, recombinantlyophilized

(Peptide N-Glycosidase F, Endoglycosidase)
Mr ca. 36.000

Concentration after reconstitution: 1000 u/µl (2.0 mg/ml) in 50 µl H2O dest.
PNGase F is a mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli. The enzyme catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F:

  • No need for refrigerated transport, storage at room temperature
  • Does not need a denaturing step
  • Works on native glycoproteins and serum glycoproteins in only minutes at room temperature
  • Digestion leads to a more complete glycan release as compared to other commercially-available enzymes
  • Especially designed and tested for mass spectrometry imaging of tissue samples

    Unit definition: Achieves complete deglycosylation of 10 µg of RNase B incubated in 1x PBS with 1 µl of PNGase F for 5 - 10 min at 37 °C or room temperature.

    HS: 35079090
    Storage Temperature: +15 °C to +30 °C

  • Cat.No.
    100 µg
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