PNGase F, recombinantsolution
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Concentration: 1000 u/µl (2.0 mg/ml), supplied in 1x PBS
PNGase F is a mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli. The enzyme catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F:
Unit definition: Achieves complete deglycosylation of 10 µg of RNase B incubated in 1x PBS with 1 µl of PNGase F for 5 - 10 min at 37 °C or room temperature.
Storage Temperature: -15 °C to -25 °C