N-(2-Hydroxyethyl)piperazine-N'-2-ethane sulfonic acidfor biochemistry

Mr 238.3 CAS [7365-45-9]

Buffering substance for biochemistry and molecular biology.
At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer has been used for a wide range of applications in biological research:

  • In tissue culture to buffer cell culture media in air
  • As a component in cell culture media like DMEM, RPMI, Hanks’ solution
  • In biological solutions for the adjustment and maintenance of pH
  • For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts
  • As a buffer component in gel filtration and affinity chromatography

  • Assay (titr.)
    A 1 cm/10 % in water
    260 nm
    280 nm
    Heavy metals (Pb)
    pH 10 % in water
    min. 99.0 %

    max. 0.1
    max. 0.08
    max. 10 ppm
    5.0 - 6.5

    1. Good, N.E. et al. (1966) Biochemistry 5, 467-77
    2. Vega, C.A. & Bates, R.G. (1976) Anal. Chem. 48,1293-6

    EINECS: 230-907-9WGK: 1LHS: 29335995
    Storage Temperature: +15 °C to +30 °C

    1 kg
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